CAJ | 학술논문

目的：总结及探讨RH Rh(D)阴性血冰冻红细胞的制备和检测方法。方法：取1U/袋的Rh Rh(D)阴性全血红细胞，采用电子摇摆称和输血器自动控制器法控制甘油加入速度。经水浴溶融化后，对冰冻红细胞进行两次洗涤，采用GPO-PAP法测定残余甘油、邻-甲联苯胺法检测上清游离血红蛋白，并计算其他指标。结果：甘油残余量6.81±2.76 g/L，上清液游离Hb0.78±0.02（g/L），体外溶血率9.96±1.24%，红细胞回收率87.68±2.89%，均要优于国家标准。结论：采用上述方法制备、融化、洗涤、检测冰冻红细胞获得了比较满意的红细胞回收率，各项质量指标也优于国家标准。
목적：총결급탐토RH Rh(D)음성혈빙동홍세포적제비화검측방법。방법：취1U/대적Rh Rh(D)음성전혈홍세포，채용전자요파칭화수혈기자동공제기법공제감유가입속도。경수욕용융화후，대빙동홍세포진행량차세조，채용GPO-PAP법측정잔여감유、린-갑련분알법검측상청유리혈홍단백，병계산기타지표。결과：감유잔여량6.81±2.76 g/L，상청액유리Hb0.78±0.02（g/L），체외용혈솔9.96±1.24%，홍세포회수솔87.68±2.89%，균요우우국가표준。결론：채용상술방법제비、융화、세조、검측빙동홍세포획득료비교만의적홍세포회수솔，각항질량지표야우우국가표준。
To summarize and discuss the preparation and detection methods of RHRh(D) negative blood frozen red blood cells. . Methods: Full red blood cells of 1 u/bag of negative Rh Rh(D) was taken. Electronic swing and donor set automatic controller controlled the glycerin to join. Frozen red blood cells were washed twice after bath solution, the GPO- PAP method measured residual glycerin, and O- methyl benzidine method detected the free hemoglobin and other indicators were calculated. Results: Glycerol residual amount was 6.81 + 2.76 g/L, supernatant free Hb0.78 + /- 0.02 (g/L), in vitro hemolysis rate 9.96 + /- 1.24%, erythrocyte recycling rate 87.68 + /- 2.89%, betterthan the national standards. Conclusion: The method above in application of melting, washing, detecting frozen erythrocyte obtains satisfactory erythrocyte recovery rate and the quality indicators are better than the national standards.