CAJ | 학술논문

目的：建立一种检测乙型耐药基因的方法。方法：采用双探针实时荧光定量聚合酶链反应(FQ-PCR)对46例HBV-DNA阳性的标本进行YMDD变异株的检测，对阳性结果进行基因测序。结果：双探针FQ- PCR法检测出YMDD变异株18例,占36.96%，18例阳性标本中拉米夫定耐药株17例、阿德福韦耐药株6例、替比夫定耐药株8例、恩替卡韦耐药株2例。结论：本法既有FQ-PCR简便、快速、灵敏、经济的特点,又有测序法对基因突变的明确性，为临床用药提供及时、准确的指导。
목적：건립일충검측을형내약기인적방법。방법：채용쌍탐침실시형광정량취합매련반응(FQ-PCR)대46례HBV-DNA양성적표본진행YMDD변이주적검측，대양성결과진행기인측서。결과：쌍탐침FQ- PCR법검측출YMDD변이주18례,점36.96%，18례양성표본중랍미부정내약주17례、아덕복위내약주6례、체비부정내약주8례、은체잡위내약주2례。결론：본법기유FQ-PCR간편、쾌속、령민、경제적특점,우유측서법대기인돌변적명학성，위림상용약제공급시、준학적지도。
To establish a method of hepatitisB resistance genes detection.MethodsSpecimens of 46 cases of HBV-DNA positivewere detected for YMDD mutation withdouble probe real-time fluorescence quantitative polymerase chain reaction (FQ-PCR),whilegene sequencing was carried out on the positive results.Results:18 cases of YMDD mutation positivewere detected with double probe FQ-PCR method with the account of 36.96%. 17 cases oflamivudine resistance were carried out withgene sequencing in the 18 cases ,aswell as6cases of Adefovirresistance, 8cases of Telbivudineresistance and 2cases of Entecavirresistance .Conclusion:It has not only the characteristics ofrapid, sensitive and economic asFQ-PCR,but also the clarity forgene sequencing.It mayprovide the rapid and accurateguidanceforclinical medicine.