医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2014年
21期
126-127
,共2页
阴道细菌%细菌培养%PCR%革兰染色法
陰道細菌%細菌培養%PCR%革蘭染色法
음도세균%세균배양%PCR%혁란염색법
Vaginal bacteria%Bacterial culture%PCR%Gram staining
目的：比较三种不同检验方法对阴道细菌的阳性检出率。方法采集本院收治的86例阴道疾病患者的阴道分泌物,分别采取细菌培养法、革兰染色法及PCR法检验阴道分泌物,并将检验结果进行对比分析。结果革兰染色法检验总阳性检出率为75%,PCR法检验总阳性检出率为97.06%；PCR法检验结果与细菌培养法无统计学差异性,>0.05；革兰染色法检验结果与细菌培养法有统计学显著性差异,<0.01；PCR法与革兰染色法有统计学明显性差异,<0.05。结论PCR检验法具有较高的阳性检出率,且操作简便快捷,能够满足临床诊断需要,可于阴道细菌临床检验工作中推广应用。
目的：比較三種不同檢驗方法對陰道細菌的暘性檢齣率。方法採集本院收治的86例陰道疾病患者的陰道分泌物,分彆採取細菌培養法、革蘭染色法及PCR法檢驗陰道分泌物,併將檢驗結果進行對比分析。結果革蘭染色法檢驗總暘性檢齣率為75%,PCR法檢驗總暘性檢齣率為97.06%；PCR法檢驗結果與細菌培養法無統計學差異性,>0.05；革蘭染色法檢驗結果與細菌培養法有統計學顯著性差異,<0.01；PCR法與革蘭染色法有統計學明顯性差異,<0.05。結論PCR檢驗法具有較高的暘性檢齣率,且操作簡便快捷,能夠滿足臨床診斷需要,可于陰道細菌臨床檢驗工作中推廣應用。
목적：비교삼충불동검험방법대음도세균적양성검출솔。방법채집본원수치적86례음도질병환자적음도분비물,분별채취세균배양법、혁란염색법급PCR법검험음도분비물,병장검험결과진행대비분석。결과혁란염색법검험총양성검출솔위75%,PCR법검험총양성검출솔위97.06%；PCR법검험결과여세균배양법무통계학차이성,>0.05；혁란염색법검험결과여세균배양법유통계학현저성차이,<0.01；PCR법여혁란염색법유통계학명현성차이,<0.05。결론PCR검험법구유교고적양성검출솔,차조작간편쾌첩,능구만족림상진단수요,가우음도세균림상검험공작중추엄응용。
Objective To compare three different test methods of vaginal bacteria positive detection rate. Methods To col ect in our hospital treated 86 cases of vaginal diseases in patients with vaginal secretion, bacteria culture method were taken respectively, gram staining method and PCR method, inspection vaginal secretions, and the test results were analyzed. Results The gram staining test total positive detection rate was 75%, the PCR method test the positive detection rate of 97.06%; PCR test results and the bacterial culture method with no statistical dif erence, > 0.05; Gram staining test results and the bacterial culture method was statistically significant difference, < 0.01; PCR method and gram staining method was statistical y significant dif erence, < 0.05. Conclusion PCR test has high positive detection rate, and easy operation and quick, can meet the needs of the clinical diagnosis and application in clinical inspection work in the vaginal bacteria.
