中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
22期
21-23,24
,共4页
不同PCR扩增试剂盒%无血缘关系个体%血样DNA检验结果%差异对比
不同PCR擴增試劑盒%無血緣關繫箇體%血樣DNA檢驗結果%差異對比
불동PCR확증시제합%무혈연관계개체%혈양DNA검험결과%차이대비
Different PCR amplification kits%Individuals without genetic connection with each other%Blood sample DNA testing result%Difference comparison
目的：讨论研究Profiler PlusTM、IdentifilerTM以及Powerplex16扩增试剂盒用于检验血样DNA检验结果的差异，并研究其扩张不平衡和基因丢失现象的发生几率。方法：选取150例完全无血缘关系的个体作为研究对象并采集其血样，分别使用两种扩增试剂盒进行检验。对所得不同结果的同一对象再使用3种扩增试剂盒进行检验。将检验所得结果进行比较。结果：3种试剂盒检测的等位基因缺失率比较差异无统计学意义（P>0.05）。Profiler PlusTM检测扩张不平衡率显著高于其余两种试剂盒（P<0.05）。结论：使用PCR扩增试剂盒对血样DNA进行检测时，会出现不同位置的异常基因，可表现为基因缺失及扩增不平衡。但Profiler PlusTM检验扩增不平衡发生率显著高于其余两种试剂盒。在实际生活对血样DNA进行检测时，除需准备主要检测扩增试剂盒外，还需准备其他不同类备用试剂盒用于互相验证及对比，尽量降低基因等位缺失及扩张不平衡发生率。
目的：討論研究Profiler PlusTM、IdentifilerTM以及Powerplex16擴增試劑盒用于檢驗血樣DNA檢驗結果的差異，併研究其擴張不平衡和基因丟失現象的髮生幾率。方法：選取150例完全無血緣關繫的箇體作為研究對象併採集其血樣，分彆使用兩種擴增試劑盒進行檢驗。對所得不同結果的同一對象再使用3種擴增試劑盒進行檢驗。將檢驗所得結果進行比較。結果：3種試劑盒檢測的等位基因缺失率比較差異無統計學意義（P>0.05）。Profiler PlusTM檢測擴張不平衡率顯著高于其餘兩種試劑盒（P<0.05）。結論：使用PCR擴增試劑盒對血樣DNA進行檢測時，會齣現不同位置的異常基因，可錶現為基因缺失及擴增不平衡。但Profiler PlusTM檢驗擴增不平衡髮生率顯著高于其餘兩種試劑盒。在實際生活對血樣DNA進行檢測時，除需準備主要檢測擴增試劑盒外，還需準備其他不同類備用試劑盒用于互相驗證及對比，儘量降低基因等位缺失及擴張不平衡髮生率。
목적：토론연구Profiler PlusTM、IdentifilerTM이급Powerplex16확증시제합용우검험혈양DNA검험결과적차이，병연구기확장불평형화기인주실현상적발생궤솔。방법：선취150례완전무혈연관계적개체작위연구대상병채집기혈양，분별사용량충확증시제합진행검험。대소득불동결과적동일대상재사용3충확증시제합진행검험。장검험소득결과진행비교。결과：3충시제합검측적등위기인결실솔비교차이무통계학의의（P>0.05）。Profiler PlusTM검측확장불평형솔현저고우기여량충시제합（P<0.05）。결론：사용PCR확증시제합대혈양DNA진행검측시，회출현불동위치적이상기인，가표현위기인결실급확증불평형。단Profiler PlusTM검험확증불평형발생솔현저고우기여량충시제합。재실제생활대혈양DNA진행검측시，제수준비주요검측확증시제합외，환수준비기타불동류비용시제합용우호상험증급대비，진량강저기인등위결실급확장불평형발생솔。
To discuss and study the difference between testing results of Profiler PlusTM,IdentifilerTM and Powerplex16 amplification kits in testing blood sample DNA,and study the occurrence rate of amplification imbalance and gene deletion phenomenon.Method:150 individuals who had no genetic connection with each other were chosen as research objects,and their blood samples were collected and tested by 2 different amplification kits. Objects with 2 different results were tested again by the third kit. All testing results were compared.Result:There were no significant differences between the 3 kits on allelic gene deletion(P<0.05). Amplification imbalance rate of Profiler PlusTM was obviously higher than the other 2 kits(P<0.05). Conclusion:When testing blood sample DNA with different PCR amplification kits, there might be abnormal genes of different positions which might manifested as gene deletion and amplification imbalance. But occurrence rate of amplification imbalance of testing result of Profiler PlusTM was obviously higher than the other 2 kits. In practical life,when testing blood sample DNA,besides major amplification kits,other different sorts of spare kits should be prepared for verification and comparison to reduce occurrence rates of allelic gene deletion and amplification imbalance.
