CAJ | 학술논문

目的：探讨香烟暴露及戒烟对大鼠肺白介素-8、谷胱甘肽（GSH）及组蛋白去乙酰化酶2（HDAC2）的影响。方法：健康雄性SD大鼠40只随机分对照组、吸烟组（1月、2月组）、戒烟组（1月、2月组）。每组8只，吸烟组及戒烟组大鼠每天给予烟熏两次，吸烟组烟熏1月、2月取材；戒烟组烟熏2月后再继续饲养1月和2月后取材，分别检测支气管肺泡灌洗液（BALF）中白介素-8和GSH、肺组织HDAC2的表达。结果：比较吸烟组、戒烟组和对照组BALF中白介素-8含量升高、GSH浓度下降，戒烟后GSH渐升高，但仍不能达到正常；吸烟组肺组织HDAC2含量逐渐降低，戒烟后HDAC2又逐渐升高，但与对照组比较，仍有下降。结论：香烟暴露可诱导气道氧化应激反应，导致GSH减少，HDAC2减少，戒烟可有所改善。
목적：탐토향연폭로급계연대대서폐백개소-8、곡광감태（GSH）급조단백거을선화매2（HDAC2）적영향。방법：건강웅성SD대서40지수궤분대조조、흡연조（1월、2월조）、계연조（1월、2월조）。매조8지，흡연조급계연조대서매천급여연훈량차，흡연조연훈1월、2월취재；계연조연훈2월후재계속사양1월화2월후취재，분별검측지기관폐포관세액（BALF）중백개소-8화GSH、폐조직HDAC2적표체。결과：비교흡연조、계연조화대조조BALF중백개소-8함량승고、GSH농도하강，계연후GSH점승고，단잉불능체도정상；흡연조폐조직HDAC2함량축점강저，계연후HDAC2우축점승고，단여대조조비교，잉유하강。결론：향연폭로가유도기도양화응격반응，도치GSH감소，HDAC2감소，계연가유소개선。
To evaluate the impacting of IL-8,GSH and HDAC2 in the lung of cigarette exposure and cessation rats. Method:40 SD rats were randomly divided into control group,smoking group(January,February group), smoking cessation group(January,February group),smoking group were sacrificed at 1 month and 2 month,smoking cessation group was sacrificed when smoking cessation for 1 month and 2 months. To collect the cells in BALF and lung tissue,and detect the IL-8,GSH and HDAC2 in rats’lung tissue. Result:Compared with control group,the levels of IL-8 in smoking group and smoking cessation group increased(P<0.01),the levels of GSH and HDAC2 in smoking group and smoking cessation group decreased(P<0.01),but smoking groups level was lower than that of smoking-cessation groups. Conclusion:It shows that cigarette exposure can induce airway oxidative stress. It can decrease after quitting,but still can't back to the normal.