CAJ | 학술논문

目的：探讨丹参酮ⅡA对肝癌细胞中凋亡抑制蛋白XIAP的影响。方法：通过MTT实验、流式细胞术、细胞形态观察检测丹参酮ⅡA对细胞增殖和凋亡的影响，通过蛋白免疫印迹的方法检测丹参酮ⅡA对细胞中凋亡抑制蛋白XIAP、凋亡蛋白caspase-3以及PARP蛋白的影响。结果：MTT、细胞形态结果显示丹参酮ⅡA对SMMC-7721细胞的生长抑制作用成时间和剂量依赖性，免疫印迹结果显示，0.5μg/mL丹参酮ⅡA作用肝癌细胞24 h后，caspase-3前体、凋亡抑制蛋白XIAP表达明显下调，活化的caspase-3、剪切形式的PARP含量显著增加。结论：丹参酮ⅡA下调凋亡抑制蛋白XIAP，活化caspase-3，促进PARP剪切诱导肝癌细胞凋亡。
목적：탐토단삼동ⅡA대간암세포중조망억제단백XIAP적영향。방법：통과MTT실험、류식세포술、세포형태관찰검측단삼동ⅡA대세포증식화조망적영향，통과단백면역인적적방법검측단삼동ⅡA대세포중조망억제단백XIAP、조망단백caspase-3이급PARP단백적영향。결과：MTT、세포형태결과현시단삼동ⅡA대SMMC-7721세포적생장억제작용성시간화제량의뢰성，면역인적결과현시，0.5μg/mL단삼동ⅡA작용간암세포24 h후，caspase-3전체、조망억제단백XIAP표체명현하조，활화적caspase-3、전절형식적PARP함량현저증가。결론：단삼동ⅡA하조조망억제단백XIAP，활화caspase-3，촉진PARP전절유도간암세포조망。
To investigate the effect of inhibitor of apoptosis protein XIAP in hepatocellular carcinoma cells treated with TanshinoneⅡA.Method:Effect of TanshinoneⅡA on cell proliferation and apoptosis were determined by MTT test,flow cytometry and cell morphology observation.The effects of TanshinoneⅡA on inhibitor of apoptosis protein XIAP,apoptosis related proteins procaspase-3 and PARP protein in cells were measured by Western blot.Result:MTT,cell morphology results showed that TanshinoneⅡA inhibited the growth of SMMC-7721 cells in a dose and time dependent manner,Western blotting results showed that 0.5μg/mL tanshinoneⅡA in hepatocellular carcinoma cells after 24 h,Caspase-3 precursor,inhibitor of apoptosis protein XIAP expression were down regulated,the content of PARP,caspase-3 splicing activation increased significantly.Conclusion:Tanshinone ⅡA can down regulate XIAP protein,activate caspase-3 and promote PARP shear,and induce apoptosis of hepatocellular carcinoma cells.