中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
23期
16-20
,共5页
周游%孙伏喜%周蓉%牛常明%郭骏华
週遊%孫伏喜%週蓉%牛常明%郭駿華
주유%손복희%주용%우상명%곽준화
小窝蛋白1%TGF-β%细胞外基质%信号传导%肺纤维化
小窩蛋白1%TGF-β%細胞外基質%信號傳導%肺纖維化
소와단백1%TGF-β%세포외기질%신호전도%폐섬유화
Caveolin1%Transforming growth factor-β%Extracellular matrix%Signal conduction%Pulmonary fibrosis
目的：探讨caveolin-1通过介导TGF-β信号通道在体外调控小鼠肺细胞外基质重塑的机制研究。方法：选取cav1-/-小鼠作为动物模型设为实验组，野生型C57BL/6J小鼠设为对照组，检测肺机械性指标，测定Ⅰ型胶原纤维与弹性纤维含量及mRNA水平，检测肺血管蛋白渗出及支气管灌洗液（BAL）中细胞含量及细胞因子水平，测定TGF-β下游信号分子Smad2及ERK1/2水平。结果：与对照组比较，实验组中肺顺应性下降，僵硬度增加（P<0.05）；BAL中总蛋白及白蛋白水平和静脉注射伊文思蓝在肺组织的含量均明显增加（P<0.05）；Ⅰ型胶原纤维与弹性纤维含量及mRNA水平增加（P<0.05）；Smad2及ERK1/2水平均明显提高（P<0.05）；但BAL中细胞含量及细胞因子水平无差异。结论：小窝蛋白1通过介导TGF-β信号转导在体外上调细胞外基质重塑，促进肺纤维化发生。
目的：探討caveolin-1通過介導TGF-β信號通道在體外調控小鼠肺細胞外基質重塑的機製研究。方法：選取cav1-/-小鼠作為動物模型設為實驗組，野生型C57BL/6J小鼠設為對照組，檢測肺機械性指標，測定Ⅰ型膠原纖維與彈性纖維含量及mRNA水平，檢測肺血管蛋白滲齣及支氣管灌洗液（BAL）中細胞含量及細胞因子水平，測定TGF-β下遊信號分子Smad2及ERK1/2水平。結果：與對照組比較，實驗組中肺順應性下降，僵硬度增加（P<0.05）；BAL中總蛋白及白蛋白水平和靜脈註射伊文思藍在肺組織的含量均明顯增加（P<0.05）；Ⅰ型膠原纖維與彈性纖維含量及mRNA水平增加（P<0.05）；Smad2及ERK1/2水平均明顯提高（P<0.05）；但BAL中細胞含量及細胞因子水平無差異。結論：小窩蛋白1通過介導TGF-β信號轉導在體外上調細胞外基質重塑，促進肺纖維化髮生。
목적：탐토caveolin-1통과개도TGF-β신호통도재체외조공소서폐세포외기질중소적궤제연구。방법：선취cav1-/-소서작위동물모형설위실험조，야생형C57BL/6J소서설위대조조，검측폐궤계성지표，측정Ⅰ형효원섬유여탄성섬유함량급mRNA수평，검측폐혈관단백삼출급지기관관세액（BAL）중세포함량급세포인자수평，측정TGF-β하유신호분자Smad2급ERK1/2수평。결과：여대조조비교，실험조중폐순응성하강，강경도증가（P<0.05）；BAL중총단백급백단백수평화정맥주사이문사람재폐조직적함량균명현증가（P<0.05）；Ⅰ형효원섬유여탄성섬유함량급mRNA수평증가（P<0.05）；Smad2급ERK1/2수평균명현제고（P<0.05）；단BAL중세포함량급세포인자수평무차이。결론：소와단백1통과개도TGF-β신호전도재체외상조세포외기질중소，촉진폐섬유화발생。
To investigate the research of caveolin1 to regulate lung extracellular matrix remodeling by mediating TGF-βsignal pathway.Method:C57BL/6J cav1-/-mice were selected as experimental group,and C57BL/6J wild type mice were determined as control group.Lung mechanics index of all mice were measured,typeⅠcollagenous fibrils and elastic fiber deposition and mRNA level were assayed,lung vascular leakage and cell content&cytokines level in bronchoalveolar lavage were measured,Smad2 and ERK1/2 level regarding as TGF-βdownstream signaling molecule were evaluated.Result:Compared with the control group,the lung compliance was down-regulated,elastance and airway resistance were up-regulated in the experimental group(P<0.05).The total protein and albumin levels in BAL and intravenous injection of Evans blue content in lung tissue were significantly increased in experimental group(P<0.05). The expression of typeⅠcollagenous fibrils and elastic fiber deposition and mRNA level were increased in experimental group(P<0.05).Smad2 and ERK1/2 level were significantly improved(P<0.05),but cell content and cytokines level in BAL were no significant deviation.Conclusion:Caveolin1 can up-regulate lung extracellular matrix remodeling by mediating TGF-βsignal pathway in vitro and progress pulmonary fibrosis.
