CAJ | 학술논문

目的：检测宫颈活检组织中上皮内瘤变（CIN）及其旁组织中hTERC基因的表达情况，对比分析其阳性率的变化，试图找到基因水平上CIN手术治疗范围。方法：采用荧光原位杂交技术（FISH）检测63例宫颈活检标本中hTERC基因的表达变化情况。结果：宫颈高级别上皮内瘤变（HSIL）区、瘤变边缘≤1 mm、1 mm<瘤变边缘≤2 mm、2 mm<瘤变边缘≤3 mm以及3 mm<瘤变边缘≤4 mm的hTERC基因阳性率分别为100%、92.31%、87.18%、64.10%和30.77%，其中，HSIL与瘤变边缘2 mm以内范围各点比较差异无统计学意义（P>0.05）；HSIL与瘤变边缘2 mm以外范围各点比较差异有统计学意义（P<0.05）；宫颈低级别上皮内瘤变（LSIL）区、瘤变边缘≤1 mm范围、1 mm<瘤变边缘≤2 mm、2 mm<瘤变边缘≤3 mm以及3 mm<瘤变边缘≤4 mm的hTERC基因阳性率分别为41.67%、25.00%、4.17%、4.17%和4.17%，其中， LSIL与瘤变边缘1 mm以内范围各点比较差异无统计学意义（P>0.05），而LSIL与瘤变边缘1 mm以外范围点比较差异有统计学意义（P<0.05）。结论：活检标本中，随着距瘤变区范围的增加，hTERC基因的阳性率明显下降，其中，距瘤变边缘>2 mm和>1 mm或许可以分别作为HSIL和LSIL基因水平上（更精确意义上的）CIN的手术治疗范围。
목적：검측궁경활검조직중상피내류변（CIN）급기방조직중hTERC기인적표체정황，대비분석기양성솔적변화，시도조도기인수평상CIN수술치료범위。방법：채용형광원위잡교기술（FISH）검측63례궁경활검표본중hTERC기인적표체변화정황。결과：궁경고급별상피내류변（HSIL）구、류변변연≤1 mm、1 mm<류변변연≤2 mm、2 mm<류변변연≤3 mm이급3 mm<류변변연≤4 mm적hTERC기인양성솔분별위100%、92.31%、87.18%、64.10%화30.77%，기중，HSIL여류변변연2 mm이내범위각점비교차이무통계학의의（P>0.05）；HSIL여류변변연2 mm이외범위각점비교차이유통계학의의（P<0.05）；궁경저급별상피내류변（LSIL）구、류변변연≤1 mm범위、1 mm<류변변연≤2 mm、2 mm<류변변연≤3 mm이급3 mm<류변변연≤4 mm적hTERC기인양성솔분별위41.67%、25.00%、4.17%、4.17%화4.17%，기중， LSIL여류변변연1 mm이내범위각점비교차이무통계학의의（P>0.05），이LSIL여류변변연1 mm이외범위점비교차이유통계학의의（P<0.05）。결론：활검표본중，수착거류변구범위적증가，hTERC기인적양성솔명현하강，기중，거류변변연>2 mm화>1 mm혹허가이분별작위HSIL화LSIL기인수평상（경정학의의상적）CIN적수술치료범위。
To detect the expression of the human telomerase(hTERC)gene of cervical intraepithelial neoplasia(CIN)and its adjacent tissues in cervical biopsy samples,to contrast and analyze the change of positive rate,to try to find the scope of surgical treatment on the CIN gene level.Method:The expression of hTERC gene was detected by FISH from 63 cases cervical biopsy specimen.Result:Positive rate of expression of hTERC gene in high-grade squamous intraepithelial lesion(HSIL),the range from the edge of neoplasia to≤1 mm,1 mm<edge of neoplasia≤2 mm,2 mm<dge of neoplasia≤3 mm,3 mm<edge of neoplasia≤4 mm were 100%,92.31%,87.18%, 64.10% and 30.77% respectively. Among them,the difference of HSIL and the range from the edge of neoplasia to≤2 mm was not statistically significant(P>0.05). But the difference of HSIL and the range from the edge of neoplasia to >2 mm was statistically significant( P<0.05).Positive rate of expression of hTERC gene in low-grade squamous intraepithelial lesion(LSIL),the range from the edge of neoplasia to ≤ 1 mm,1 mm<edge of neoplasia ≤ 2 mm, 2 mm<edge of neoplasia ≤ 3 mm,3 mm<edge of neoplasia ≤ 4 mm were 41.67%,25.00%,4.17%,4.17% and 4.17%respectively. Among them,the difference of LSIL and the range from the edge of neoplasia to≤1 mm was not statistically significant( P>0.05). But the difference of LSIL and the range from the edge of neoplasia to>1 mm was statistically significant(P<0.05).Conclusion:With the increase of range from the area of neoplasia,the positive rate of hTERC gene decreases obviously. The range from HSIL edge>2 mm and LSIL edge>1 mm may be the scope of surgical treatment on the CIN gene level,respectively(a more precise sense).