中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
14期
51-52
,共2页
益阴消渴滴丸%质量标准%葛根素%荔枝核
益陰消渴滴汍%質量標準%葛根素%荔枝覈
익음소갈적환%질량표준%갈근소%려지핵
Yiyin Xiaoke Dropping Pills%quality standard%puerarin%semen litchi
目的：建立益阴消渴滴丸的定性鉴别和含量测定方法。方法用薄层色谱（TLC）法定性鉴别益阴消渴滴丸中的葛根、荔枝核；用高效液相色谱（HPLC）法测定葛根中葛根素的含量，色谱条件为迪马 C18柱(250 mm ×4.6 mm，5μm)，流动相为甲醇-水（25：75），检测波长为250 nm，柱温30℃。结果 TLC 色谱斑点清晰，分离度好，阴性对照无干扰；葛根素进样量在0.1738~1.7380μg 范围内与峰面积积分值呈良好线性关系（ r =0.9999），平均回收率为98.82%，RSD 为0.37%。结论所建立的定性、定量分析方法可用于益阴消渴滴丸的质量控制。
目的：建立益陰消渴滴汍的定性鑒彆和含量測定方法。方法用薄層色譜（TLC）法定性鑒彆益陰消渴滴汍中的葛根、荔枝覈；用高效液相色譜（HPLC）法測定葛根中葛根素的含量，色譜條件為迪馬 C18柱(250 mm ×4.6 mm，5μm)，流動相為甲醇-水（25：75），檢測波長為250 nm，柱溫30℃。結果 TLC 色譜斑點清晰，分離度好，陰性對照無榦擾；葛根素進樣量在0.1738~1.7380μg 範圍內與峰麵積積分值呈良好線性關繫（ r =0.9999），平均迴收率為98.82%，RSD 為0.37%。結論所建立的定性、定量分析方法可用于益陰消渴滴汍的質量控製。
목적：건립익음소갈적환적정성감별화함량측정방법。방법용박층색보（TLC）법정성감별익음소갈적환중적갈근、려지핵；용고효액상색보（HPLC）법측정갈근중갈근소적함량，색보조건위적마 C18주(250 mm ×4.6 mm，5μm)，류동상위갑순-수（25：75），검측파장위250 nm，주온30℃。결과 TLC 색보반점청석，분리도호，음성대조무간우；갈근소진양량재0.1738~1.7380μg 범위내여봉면적적분치정량호선성관계（ r =0.9999），평균회수솔위98.82%，RSD 위0.37%。결론소건립적정성、정량분석방법가용우익음소갈적환적질량공제。
Objective To establish the methods of qualitative identification and content determination in Yiyin Xiaoke Dropping Pills. Methods The TLC method was used to qualitatively identify Radix puerariae and Semen litchi;and the content of puerarin was determined by HPLC. The chromatographic parameters was the Diamonsil C18 column(250 mm × 4. 6 mm,5 μm),methanol - water(25 : 75) as the mobile phase,the detection wavelength of 250 nm and the column temperature was 30 ℃. Results The developed TLC sports were clear with good separation and without negative interference;The linear range of puerarin were 0. 173 8 - 1. 738 0μg( r = 0. 999 9). The average recovery rate was 98. 82% ,RSD = 0. 37% . Conclusion The established qualitative and quantitative analysis method is applica-ble for the quality control of Yiyin Xiaoke Dropping Pills.
