CAJ | 학술논문

目的：筛选玉簪花总多糖的最佳提取工艺并建立测定其含量的方法。方法通过单因素试验及正交试验设计考察玉簪花总多糖水提醇沉的制备条件，以葡萄糖标准液作为考察指标绘制标准曲线，苯酚-硫酸法测定玉簪花总多糖的含量。结果玉簪花总多糖的最佳提取工艺为加30倍量水，提取温度80℃，浸提3次，每次3 h；测定样品的玉簪花总多糖含量达3.65%。结论优选工艺合理、可行，含量测定方法准确、简便，具有良好的重现性。
목적：사선옥잠화총다당적최가제취공예병건립측정기함량적방법。방법통과단인소시험급정교시험설계고찰옥잠화총다당수제순침적제비조건，이포도당표준액작위고찰지표회제표준곡선，분분-류산법측정옥잠화총다당적함량。결과옥잠화총다당적최가제취공예위가30배량수，제취온도80℃，침제3차，매차3 h；측정양품적옥잠화총다당함량체3.65%。결론우선공예합리、가행，함량측정방법준학、간편，구유량호적중현성。
Objective To screen the optimal extraction technology of total polysaccharide from flos hosta plantaginea and to establish its content determination method. Methods The single factor experiment and the orthogonal experiment were adopted to investigate the preparation conditions by the water extraction and alchol precipitation, the glucose standard solution was taken as the reference index for drawing the standard curve. The phenol - sulfurric acid colorimetry was adotd to determine the total polysaccharide content in flos hosta plantaginea. Results The optimum extraction technology was adding 30 times the amount of water, the extracting temperature of 80 ℃ , extracting for 3 times, 3 h for each time, the total polysaccharide content in the detected sample reached 3. 65% . Conclusion The op-timized extraction technology is rational and feasible, the content determination method is accurate and simple with good reproducibility.