CAJ | 학술논문

目的：建立测定心脑欣片中甜菜碱含量的方法。方法采用液相色谱-串联质谱联用（LC - MS / MS）法测定甜菜碱的含量，以电喷雾离子源(ESI)正离子模式将样品离子化，多反应监测(MRM)模式下对甜菜碱( m / z 118.20→57.92和118.20→59.00)进行测定。色谱柱为 Venusil HILIC 柱(100 mm ×2.1 mm，3μm），流动相为乙腈-水(80：20)，流速为0.3 mL / min。结果甜菜碱进样质量浓度在4.44~177.6 ng / mL 范围内与峰面积积分值线性关系良好（ r =0.9999），平均回收率为99.45%，RSD 为1.43%（ n =6）。结论该方法简便、可靠、准确，可作为测定心脑欣片中甜菜碱含量的方法。
목적：건립측정심뇌흔편중첨채감함량적방법。방법채용액상색보-천련질보련용（LC - MS / MS）법측정첨채감적함량，이전분무리자원(ESI)정리자모식장양품리자화，다반응감측(MRM)모식하대첨채감( m / z 118.20→57.92화118.20→59.00)진행측정。색보주위 Venusil HILIC 주(100 mm ×2.1 mm，3μm），류동상위을정-수(80：20)，류속위0.3 mL / min。결과첨채감진양질량농도재4.44~177.6 ng / mL 범위내여봉면적적분치선성관계량호（ r =0.9999），평균회수솔위99.45%，RSD 위1.43%（ n =6）。결론해방법간편、가고、준학，가작위측정심뇌흔편중첨채감함량적방법。
Objective To establish the method for determining the betaine contents in Xinnaoxin Tablet. Methods The betaine content in Xinnaoxin Tablet was determined by LC - MS / MS. The sample was ionized by the eletrospray ionization(ESI) positive ion mode and betaine( m / z 118. 20→57. 92 and 118. 20→59. 00) was detected under the multiple reaction monitor(MRM) mode. The chromatographic column was the Venusil HILIC column(100 mm × 2. 1 mm × 3 μm) . The mobile phase was acetonitrile - water(80 : 20), the flow rate was 0. 3 mL / min. Results The mass concentration of betaine sample size in the range of 4. 44 - 177. 6 ng / mL exhibited the good linear relation( r = 0. 999 9). The average recovery rate was 99. 45% , RSD 1. 43% ( n = 6). Conclusion This method is simple, reliable and ac-curate, which can be used to determine the betaine content in Xinnaoxin Tablet.