CAJ | 학술논문

目的：探讨局部应用纤维蛋白凝胶携载血管内皮生长因子，对损伤坐骨神经再生的影响，为临床药物治疗坐骨神经损伤提供实验依据。方法将36只Wistar大鼠左侧坐骨神经切断，神经两断端原位缝合，制作大鼠坐骨神经损伤动物模型。然后将大鼠随机分成实验组（纤维蛋白凝胶携载血管内皮生长因子给药组）与对照组（血管内皮生长因子基因转染给药组），每组18只，于用药后8、12周行肉眼观察、光镜观察，图像分析仪行神经断面分析，测轴突直径、髓鞘厚度和有髓神经纤维数目。结果实验组8周的轴突直径、髓鞘厚度和有髓神经纤维数目和对照组相比差异有统计学意义（P＜0.05），12周时2组差异无统计学意义（P＞0.05）。结论纤维蛋白凝胶可以作为血管内皮生长因子的载体。纤维蛋白凝胶携载血管内皮生长因子给药可以促进损伤神经结构和功能的恢复。
목적：탐토국부응용섬유단백응효휴재혈관내피생장인자，대손상좌골신경재생적영향，위림상약물치료좌골신경손상제공실험의거。방법장36지Wistar대서좌측좌골신경절단，신경량단단원위봉합，제작대서좌골신경손상동물모형。연후장대서수궤분성실험조（섬유단백응효휴재혈관내피생장인자급약조）여대조조（혈관내피생장인자기인전염급약조），매조18지，우용약후8、12주행육안관찰、광경관찰，도상분석의행신경단면분석，측축돌직경、수초후도화유수신경섬유수목。결과실험조8주적축돌직경、수초후도화유수신경섬유수목화대조조상비차이유통계학의의（P＜0.05），12주시2조차이무통계학의의（P＞0.05）。결론섬유단백응효가이작위혈관내피생장인자적재체。섬유단백응효휴재혈관내피생장인자급약가이촉진손상신경결구화공능적회복。
Objective To investigate the effects of exogenous vascular endothelial growth factor (VEGF) mixed with fibrin glue (FG) as a carrier on nerve regeneration in repairing rat sciatic nerve injury .Methods The rat sciatic nerve injury model was made by cutting off the left sciatic nerve of rat and suturing in normal position . Thirty-six Wistar rats were randomly divided into experimental group (VEGF mixed with fibrin glue group ) and control group( gene transfection group) ,with 18 in each .Gross appearance ,light microscopic observation ,nerve sectional image analysis were performed at 8 and 12 weeks after medication to measure the mean diameter of neuraxis ,thickness of myelin sheath and total number of myelinated fiber .Results There was significant devia-tion in the mean diameter of neuraxis ,thickness of myelin sheath and total number of myelinated fiber at 8 week after medication compared with the control group (P<0.05) and no significant deviation at 12 week (P>0.05) . Conclusion Fibrin glue can be used as a carrier for VEGF to repair injured sciatic nerves .These results indicate locally VEGF mixed with fibrin glue can exert nerve regeneration in the injured sciatic nerve area and enhance neu-rological function recovery of the sciatic nerve injured rats .