CAJ | 학술논문

目的研究新型NRG1依赖性HER3单克隆抗体对HER2过表达N87胃癌细胞Lapatinib耐药的逆转作用。方法 HER2过度表达N87细胞接受不同浓度Lapatinib和/或NRG1依赖HER3单克隆抗体（3D4B3）处理后，利用CellTiter 96 Aqueous单溶液细胞增殖检测试剂盒检测细胞活力，利用BrdU掺入法检测细胞的增殖与凋亡。结果无NRG1刺激，Lapatinib对HER2过表达N87细胞增殖有显著抑制作用，而3D4B3几乎无抑制作用，联合应用无叠加效应。随着NRG1刺激和HER3的激活，Lapatinib的增殖抑制效应明显减弱或消失，Lapatinib联合3D4B3再次有效抑制N87细胞的增殖。结论3D4B3可能通过抑制HER2过表达N87胃癌细胞增殖部分逆转Lapatinib的耐药。
목적연구신형NRG1의뢰성HER3단극륭항체대HER2과표체N87위암세포Lapatinib내약적역전작용。방법 HER2과도표체N87세포접수불동농도Lapatinib화/혹NRG1의뢰HER3단극륭항체（3D4B3）처리후，이용CellTiter 96 Aqueous단용액세포증식검측시제합검측세포활력，이용BrdU참입법검측세포적증식여조망。결과무NRG1자격，Lapatinib대HER2과표체N87세포증식유현저억제작용，이3D4B3궤호무억제작용，연합응용무첩가효응。수착NRG1자격화HER3적격활，Lapatinib적증식억제효응명현감약혹소실，Lapatinib연합3D4B3재차유효억제N87세포적증식。결론3D4B3가능통과억제HER2과표체N87위암세포증식부분역전Lapatinib적내약。
Objective This research focused on that new NRG1/HER3 monoclonal antibody(3D4B3)reversed lapatinib resistance in HER2 over-expression gastric cancer N87 cells. Methods After HER2 over -expression N87 cells received lapatinib and/or NRG1 -dependent 3D4B3 treatment,cell viability was detected using celltiter 96 aqueous one solution cell proliferation assay method,and cell proliferation and apop-tosis were detected using BrdU incorporation method. Results Without NRG1 stimulation,lapatinib had significant proliferation inhibition on HER2 over-expression N87 cells,3D4B3 had no significant inhibitory effect,and combined application had no superimposed effect. With NRG1 stimulation and the activation of HER3,the proliferation inhibitory effects of lapatinib were obviously weakened or disappeared. The combination of NRG1 dependent 3D4B3 with lapatinib effectively inhibited N87 cells proliferation again. Conclusion NRG1 dependent 3D4B3 could partly re-versed lapatinib resistance by inhibiting N87 cells proliferation.