中国骨与关节外科
中國骨與關節外科
중국골여관절외과
CHINESE BONE AND JOINT SURGERY
2014年
3期
235-240
,共6页
刘晓阳%邱贵兴%翁习生%吴志宏%于斌%王以朋
劉曉暘%邱貴興%翁習生%吳誌宏%于斌%王以朋
류효양%구귀흥%옹습생%오지굉%우빈%왕이붕
特发性脊柱侧凸%青少年%长链非编码RNA
特髮性脊柱側凸%青少年%長鏈非編碼RNA
특발성척주측철%청소년%장련비편마RNA
idiopathic scoliosis%adolescent%long non-coding RNA
背景：长链非编码RNA（lncRNAs）是真核细胞中一类长度超过200核苷酸的非编码RNA分子，与人类众多疾病的发生有密切的关系。然而，lncRNAs在青少年特发性脊柱侧凸（AIS）的表达情况尚不清楚。目的：利用基因芯片筛选AIS患者外周血中差异表达的lncRNAs和mRNAs，分析lncRNAs在AIS发病中的可能作用。方法：选取2013年北京协和医院就诊的20例AIS患者和20例正常对照。利用Agilent human lncRNA+mRNA Array V3.0微阵列芯片检测4例AIS患者和4例年龄匹配的正常对照的lncRNAs和mRNAs表达，对差异表达的mRNAs进行GO、Pathway分析，构建lncRNAs和mRNAs的共表达网络，预测lncRNAs的可能调控靶点。结果：AIS患者中差异表达的lncRNAs有139条，差异表达的mRNAs有546条。GO分析发现，差异表达的mRNAs产物主要参与蛋白结合、金属离子结合、核苷酸结合、调节转录、RNA剪切等。差异表达的mRNAs主要参与细胞黏附分子、Wnt通路、Toll样受体通路、MAPK通路等。靶基因预测，7条lncRNAs可能通过调节mRNAs的表达参与了AIS的发病。结论：本研究发现了AIS患者外周血中差异表达的lncRNAs和mRNAs。lncRNAs可能通过调控mRNA的表达参与AIS的发病或发展。
揹景：長鏈非編碼RNA（lncRNAs）是真覈細胞中一類長度超過200覈苷痠的非編碼RNA分子，與人類衆多疾病的髮生有密切的關繫。然而，lncRNAs在青少年特髮性脊柱側凸（AIS）的錶達情況尚不清楚。目的：利用基因芯片篩選AIS患者外週血中差異錶達的lncRNAs和mRNAs，分析lncRNAs在AIS髮病中的可能作用。方法：選取2013年北京協和醫院就診的20例AIS患者和20例正常對照。利用Agilent human lncRNA+mRNA Array V3.0微陣列芯片檢測4例AIS患者和4例年齡匹配的正常對照的lncRNAs和mRNAs錶達，對差異錶達的mRNAs進行GO、Pathway分析，構建lncRNAs和mRNAs的共錶達網絡，預測lncRNAs的可能調控靶點。結果：AIS患者中差異錶達的lncRNAs有139條，差異錶達的mRNAs有546條。GO分析髮現，差異錶達的mRNAs產物主要參與蛋白結閤、金屬離子結閤、覈苷痠結閤、調節轉錄、RNA剪切等。差異錶達的mRNAs主要參與細胞黏附分子、Wnt通路、Toll樣受體通路、MAPK通路等。靶基因預測，7條lncRNAs可能通過調節mRNAs的錶達參與瞭AIS的髮病。結論：本研究髮現瞭AIS患者外週血中差異錶達的lncRNAs和mRNAs。lncRNAs可能通過調控mRNA的錶達參與AIS的髮病或髮展。
배경：장련비편마RNA（lncRNAs）시진핵세포중일류장도초과200핵감산적비편마RNA분자，여인류음다질병적발생유밀절적관계。연이，lncRNAs재청소년특발성척주측철（AIS）적표체정황상불청초。목적：이용기인심편사선AIS환자외주혈중차이표체적lncRNAs화mRNAs，분석lncRNAs재AIS발병중적가능작용。방법：선취2013년북경협화의원취진적20례AIS환자화20례정상대조。이용Agilent human lncRNA+mRNA Array V3.0미진렬심편검측4례AIS환자화4례년령필배적정상대조적lncRNAs화mRNAs표체，대차이표체적mRNAs진행GO、Pathway분석，구건lncRNAs화mRNAs적공표체망락，예측lncRNAs적가능조공파점。결과：AIS환자중차이표체적lncRNAs유139조，차이표체적mRNAs유546조。GO분석발현，차이표체적mRNAs산물주요삼여단백결합、금속리자결합、핵감산결합、조절전록、RNA전절등。차이표체적mRNAs주요삼여세포점부분자、Wnt통로、Toll양수체통로、MAPK통로등。파기인예측，7조lncRNAs가능통과조절mRNAs적표체삼여료AIS적발병。결론：본연구발현료AIS환자외주혈중차이표체적lncRNAs화mRNAs。lncRNAs가능통과조공mRNA적표체삼여AIS적발병혹발전。
Background: Long noncoding RNAs (lncRNAs) are broadly classified as transcripts longer than 200 nucleotides, which function in a wide range of diseases. Expression profile of lncRNAs in AIS was still unclear. Objective:To detect differentially expressed lncRNAs and mRNAs in peripheral blood samples from AIS patients using mi-croarray and explore the role of lncRNA in the pathogenesis of AIS. Methods:A total of 20 AIS patients from Peking Union Medical College Hospital were recruited as cases together with 20 healthy controls. Peripheral blood was collected from 4 patients with AIS and 4 age-matched normal children and tested with Agilent human lncRNA+mRNA Array V3.0. GO and Pathway analysis was performed. The coding-non-coding gene co-expression network was constructed based on the correlation analysis. Target regulated by lncRNAs was predicted with bioinformatic prediction. Results:A total of 139 deregulated lncRNAs and 546 deregulated mRNAs were detected in AIS patients.GO Term enrich-ment in the differentially expressed mRNA list included protein binding, metal ion binding, nucleotide binding, regulation of transcription, RNA splicing et al. Differentially expressed mRNAs may involve in Cell adhesion molecules, Wnt signal-ing pathway, Toll-like receptor signaling pathway, MAPK signaling pathway and so on. Seven lncRNAs may regulate mRNAs expression in pathogenesis of AIS. Conclusions:This is the first time to find lncRNAs and mRNAs expression in AIS patients using microarray. Differentially expressed lncRNAs may play a role in the pathogenesis of AIS.
