CAJ | 학술논문

利用ISSR分子标记技术，对来自国内外的33个青花菜（Brassica oleracea var．italica）材料进行遗传多样性研究。从100条通用ISSR引物中筛选出15条用于PCR扩增，共得到可重复的211条清晰谱带，其中多态性谱带180条，多态性条带比率（ PPB）为85.31％，平均Nei's基因多样性指数与Shannon's信息指数为0.3327和0.4877；材料间遗传距离范围为0.09～0.45，平均0.28；聚类分析表明，33份青花菜种质可分为4组，没有明显的地域分布规律。结果表明，ISSR标记可用于青花菜种质的分子鉴定；青花菜供试材料具有一定的遗传多样性。
이용ISSR분자표기기술，대래자국내외적33개청화채（Brassica oleracea var．italica）재료진행유전다양성연구。종100조통용ISSR인물중사선출15조용우PCR확증，공득도가중복적211조청석보대，기중다태성보대180조，다태성조대비솔（ PPB）위85.31％，평균Nei's기인다양성지수여Shannon's신식지수위0.3327화0.4877；재료간유전거리범위위0.09～0.45，평균0.28；취류분석표명，33빈청화채충질가분위4조，몰유명현적지역분포규률。결과표명，ISSR표기가용우청화채충질적분자감정；청화채공시재료구유일정적유전다양성。
The genetic diversity of 33 broccoli ( Brassica oleracea var.italica) germplasm resources , from home and abroad, was investigated using ISSR molecular marker method .Fifteen primers were selected from 100 universal ones , and they produced 211 distinctive bands , including 180 polymorphic sites with 85.31%of polymorphic bands . The average Nei's genetic diversity index and Shannon's information index were 0.332 7 and 0.487 7 , respectively . The genetic distance ranged from 0.09 to 0.45 with an average value of 0.28 .Cluster analysis divided the 33 broccoli materials into 4 groups, and showed no obvious correlation with geographic distribution .The results revealed that the ISSR markers could be used to identify broccoli germplasm resources , and the materials tested showed a certain level of genetic diversity .