CAJ | 학술논문

制备抗沙眼衣原体（ CT）单克隆抗体，建立沙眼衣原体的胶体金免疫层析快速检测方法。采用纯化沙眼衣原体主要外膜蛋白（ MOMP）免疫Balb/c小鼠，选取高效价的免疫小鼠脾细胞与骨髓瘤细胞融合， ELISA检测筛选分泌抗MOMP单克隆抗体（ McAb）的细胞株并进行相关鉴定。共获得分泌IgG1，IgG2b和IgM的3株单克隆抗体杂交瘤细胞株。纯化的单克隆抗体与鹦鹉热衣原体、肺炎衣原体、肺炎支原体均无交叉反应。通过位点分析选择配对良好的两株McAb，制备检测CT的双抗体夹心法免疫层析试纸条。制备的胶体金试纸条对MOMP的最低检出值可达50 ng· mL-1。
제비항사안의원체（ CT）단극륭항체，건립사안의원체적효체금면역층석쾌속검측방법。채용순화사안의원체주요외막단백（ MOMP）면역Balb/c소서，선취고효개적면역소서비세포여골수류세포융합， ELISA검측사선분비항MOMP단극륭항체（ McAb）적세포주병진행상관감정。공획득분비IgG1，IgG2b화IgM적3주단극륭항체잡교류세포주。순화적단극륭항체여앵무열의원체、폐염의원체、폐염지원체균무교차반응。통과위점분석선택배대량호적량주McAb，제비검측CT적쌍항체협심법면역층석시지조。제비적효체금시지조대MOMP적최저검출치가체50 ng· mL-1。
To prepare monoclonal antibodies ( McAb ) against Chlamydia trachomatis ( CT ) and establish the Chlamydia trachomatis GICA rapid detection method .Balb/c mice were injected with purified major outer membrane protein ( MOMP) of Chlamydia trachomatis.Spleen cells from immunized mice were fused with SP 2/0 myeloma cells.Hybirdoma cells which secrete antibodies against MOMP were selected and then identified .Three monoclonal antibodies which had very high affinity to MOMP were obtained .The isotypes of McAbs were IgG 1 , IgG2b and IgM. The above McAbs had no cross-reaction with Chlamydia psittaci, Chlamydia pneumoniae and Mycoplasma pneumoniae.Two McAbs with good pairing were selected to prepare GICA strip .The strip made up of these McAbs can be applied to detect CT .The sensitivity by GICA strip for detecting MOMP was about 50 ng· mL-1 .