CAJ | 학술논문

无论是用作牲畜饲料或榨汁做糖，茎秆汁液含量对于甜高粱而言都是一个非常重要的农艺性状。本研究通过对两套独立的F2群体观察统计发现，影响高粱茎秆汁液含量（ MCS）的基因Dd呈现质量性状基因的遗传规律，且干髓对多汁为显性。用SSR标记初定位表明该基因位于第6号染色体，扩大定位群体后，用群体F2-1将其定位在标记sm06068和sm06093之间，用群体F2-2将其定位在sm06068和sm06069之间，两个区段的物理距离均约为470 kb。为了进一步缩小定位区间，对定位群体的亲本进行了测序分析并开发了2个InDel标记。最终，目标基因被锁定在只包含6个候选基因的区间范围内。基因d的精细定位为该基因的克隆及今后分子育种中的应用打下基础。
무론시용작생축사료혹자즙주당，경간즙액함량대우첨고량이언도시일개비상중요적농예성상。본연구통과대량투독립적F2군체관찰통계발현，영향고량경간즙액함량（ MCS）적기인Dd정현질량성상기인적유전규률，차간수대다즙위현성。용SSR표기초정위표명해기인위우제6호염색체，확대정위군체후，용군체F2-1장기정위재표기sm06068화sm06093지간，용군체F2-2장기정위재sm06068화sm06069지간，량개구단적물리거리균약위470 kb。위료진일보축소정위구간，대정위군체적친본진행료측서분석병개발료2개InDel표기。최종，목표기인피쇄정재지포함6개후선기인적구간범위내。기인d적정세정위위해기인적극륭급금후분자육충중적응용타하기출。
The moisture content is very important for both forage and syrup use of sweet sorghum .In the present study, the gene Dd, which is related to moisture content of stem ( MCS), was proved as qualitative and the dominance of pithy over juicy type by the genetic analysis , using two F 2 populations derived from a pithy cultivar Fangshannian crossing a juicy cultivar Shuangli and a juicy cultivar Btx 623 crossing a pithy cultivar 7361 .Primary mapping of the gene indicated that d was located on chromosome 6.By enlarging the mapping population , the locus was then fine mapped to a 470 kb interval between SSR markers sm 06068 and sm06093 for F2-1 and sm06068 and sm06069 for F2-2.To narrow down the number of candidate genes , the target regions of Fangshannian , Shuangli and 7361 were sequenced and 2 InDel markers were mined .Finally, the locus was located to the region between markers sm06068 and Sb6-2, which harbored only six candidate genes .Genetic mapping of d provided an opportunity to identify genes involved in the moisture content of sorghum .