CAJ | 학술논문

目的：建立广升麻的HPLC指纹图谱，为广升麻的质量控制提供依据。方法：采用高效液相色谱法，以β-蜕皮甾酮为内参照物，采用色谱柱Agilent C18（3.0 mm ×100 mm，1.8μm），乙腈-0.1％甲酸溶液梯度洗脱，流量为0.4 mL· min-1，检测波长为310 nm，柱温30℃。结果：获得了较为理想的广升麻药材HPLC指纹图谱，确定了13个共有峰。结论：该方法简便、准确、可靠，为评价广升麻药材的质量提供依据。
목적：건립엄승마적HPLC지문도보，위엄승마적질량공제제공의거。방법：채용고효액상색보법，이β-세피치동위내삼조물，채용색보주Agilent C18（3.0 mm ×100 mm，1.8μm），을정-0.1％갑산용액제도세탈，류량위0.4 mL· min-1，검측파장위310 nm，주온30℃。결과：획득료교위이상적엄승마약재HPLC지문도보，학정료13개공유봉。결론：해방법간편、준학、가고，위평개엄승마약재적질량제공의거。
Objective:To establish HPLC fingerprint of Radix Serratulae Chinensis.Methods: β-ecdysone was used as the internal standard substance.The reversed phase HPLC system was consisted of a C18 column (3.0 mm ×100 mm,1.8 μm) and a mixture of acetonitrile-0.1%formic acid solution as the mobile phase with gradient elution mode at the flow rate of 0.4 mL· min-1.The absor-bance was monitored at 310 nm and the column temperature was 30℃.Results:A satisfactory method for HPLC fingerprint determina-tion of Radix Serratulae Chinensis was established , 13 characteristic peaks in the HPLC fingerprint were indicated.Conclusion:The method is simple , accurate with good repeatability and can be used as a quality control method for Radix Serratulae Chinensis .