CAJ | 학술논문

目的：探讨桉叶油对维甲酸（RA）致畸胎大鼠四肢骨骼发育的影响。方法 SD 孕大鼠42只，随机分为6组，每组7只，正常对照组、RA 组、溶剂对照组（花生油+ RA）及桉叶油100，200和300 mg·kg-1+RA 组。溶剂对照组于孕第7~14天灌胃给予花生油，每只2 mL，每天1次，孕第10天灌胃1次 RA 40 mg·kg-1。桉叶油3个剂量组于孕第7~14天分别灌胃给予桉叶油，每天1次，孕第10天灌胃1次RA 40 mg·kg-1。RA 组于孕第10天灌胃1次 RA 40 mg·kg-1。各组于孕21 d 处死孕鼠取胚胎，测量胎鼠体质重、身长和尾长。每窝活胎随机提取1/2~1/3胎鼠用阿利新蓝和茜素红进行骨骼染色，体视镜下观察骨骼发育，测量四肢长骨长度和骨化长度。结果 RA 灌胃各组胎鼠的体质量、身长和尾长均明显低于正常对照组（P﹤0.05，P﹤0.01）；桉叶油各组胎鼠体质量和身长明显高于 RA 组（P﹤0.05，P﹤0.01）；溶剂对照组胎鼠体质量、身长和尾长与 RA 组比较无差异；桉叶油各组胎鼠尾长与 RA 组比较无明显变化。RA 各组胎鼠四肢骨的骨长度和骨化长度均低于正常对照组（P﹤0.05，P﹤0.01）；桉叶油各组四肢骨骨长度均高于RA 组和溶剂对照组（P﹤0.05，P﹤0.01），溶剂对照组四肢骨骨长度与 RA 组比较无差异；桉叶油各组四肢骨骨化长度高于 RA 组和溶剂对照组（P﹤0.05，P﹤0.01），溶剂对照组四肢骨骨化长度与 RA 组比较无差异。结论桉叶油对 RA 引起胎鼠四肢骨骨骼发育迟缓和骨化迟缓有一定的拮抗作用。
목적：탐토안협유대유갑산（RA）치기태대서사지골격발육적영향。방법 SD 잉대서42지，수궤분위6조，매조7지，정상대조조、RA 조、용제대조조（화생유+ RA）급안협유100，200화300 mg·kg-1+RA 조。용제대조조우잉제7~14천관위급여화생유，매지2 mL，매천1차，잉제10천관위1차 RA 40 mg·kg-1。안협유3개제량조우잉제7~14천분별관위급여안협유，매천1차，잉제10천관위1차RA 40 mg·kg-1。RA 조우잉제10천관위1차 RA 40 mg·kg-1。각조우잉21 d 처사잉서취배태，측량태서체질중、신장화미장。매와활태수궤제취1/2~1/3태서용아리신람화천소홍진행골격염색，체시경하관찰골격발육，측량사지장골장도화골화장도。결과 RA 관위각조태서적체질량、신장화미장균명현저우정상대조조（P﹤0.05，P﹤0.01）；안협유각조태서체질량화신장명현고우 RA 조（P﹤0.05，P﹤0.01）；용제대조조태서체질량、신장화미장여 RA 조비교무차이；안협유각조태서미장여 RA 조비교무명현변화。RA 각조태서사지골적골장도화골화장도균저우정상대조조（P﹤0.05，P﹤0.01）；안협유각조사지골골장도균고우RA 조화용제대조조（P﹤0.05，P﹤0.01），용제대조조사지골골장도여 RA 조비교무차이；안협유각조사지골골화장도고우 RA 조화용제대조조（P﹤0.05，P﹤0.01），용제대조조사지골골화장도여 RA 조비교무차이。결론안협유대 RA 인기태서사지골골격발육지완화골화지완유일정적길항작용。
OBJECTlVE To study the effect of eucaIyptus oiI on fetus skeIetaI deveIopment of retin-oic acid( RA)induced teratogenic rats. METHODS Forty-two pregnant SD rats were randomIy and equaIIy divided into 6 groups:eucaIyptus oiI 100,200 and 300 mg·kg-1+RA groups,soIvent group(peanut oiI+RA)group,RA group and normaI group with normaI diet. Three eucaIyptus oiI and soIvent groups were ig given eucaIyptus oiI in 100,200,and 300 mg·kg-1 and peanut oiI 2 mL at the 7th to 14th day of gestation,respectiveIy,and RA 40 mg·kg-1 at the 10th day of gestation. RA group were ig given RA 40 mg·kg-1 at the 10th day of gestation. AII pregnant rats were sacrificed at the 21th day. The Iength of fetus body and taiI as weII as body mass of the embryos were measured. 1/ 2-1/ 3 fetaI rats from each Iitter were randomIy extracted to observe skeIetaI deveIopment under a stereoscope by staining with aIcian bIue and aIizarin red S. The Iength of Iimbs and bone ossification was measured. RESULTS The fetus body mass,body Iength,taiI Iength of normaI group significantIy surpassed those in the other groups(P﹤0.05,P﹤0.01). The fetus body mass and body Iength of each eucaIyptus oiI group were significantIy better than RA group(P﹤0.05,P﹤0.01). The fetus body mass,body Iength and taiI Iength of soIvent group were not significantIy different from those in the RA group. The fetus taiI Iength in eucaIyptus oiI groups had no significant difference compared with RA group. The Iimb and bone ossification of normaI group were Ionger than in RA group(P﹤0.05,P﹤0.01). The Iimb Iength of the eucaIyptus oiI groups was Ionger than in RA group and soIvent controI group(P﹤0.05,P﹤0.01),but there was no difference between the soIvent controI group and RA group. The Iimb ossification of each eucaIyptus oiI group was aIso Ionger than that in RA group and soIvent controI group(P﹤0.05,P﹤0.01),but there was no difference between the soIvent controI group and RA group. CONCLUSlON EucaIyptus oiI can antagonize the retardation of skeIetaI growth and ossification of fetaI rats caused by RA.