中国药理学与毒理学杂志
中國藥理學與毒理學雜誌
중국약이학여독이학잡지
CHINESE JOURNAL OF PHARMACOLOGY AND TOXICOLOGY
2014年
4期
515-518
,共4页
二甲基甲酰胺%维生素 E%肝损伤
二甲基甲酰胺%維生素 E%肝損傷
이갑기갑선알%유생소 E%간손상
dimethyIformamide%vitamin E%Iiver damage
目的：探讨维生素 E（Vit E）对二甲基甲酰胺（DmF）致小鼠慢性肝损伤的预防作用。方法将40只健康 SPF 级雄性昆明小鼠随机分为正常对照组、Vit E 对照组、DmF 染毒模型组和 DmF+Vit E 干预组，每组10只。DmF 染毒模型组和 DmF+Vit E 干预组 ig 给予 DmF 0.5 g·kg-1染毒；2 h 后，Vit E 对照组和 DmF+Vit E 干预组 ig 给予 Vit E 5 mg·kg-1，每天1次，连续30 d。用试剂盒测定小鼠血清谷草转氨酶（GOT）、谷丙转氨酶（GPT）和黄嘌呤氧化酶（XOD）活性及丙二醛（mDA）含量，HE 染色法观察肝组织病理改变。结果与正常对照组比较，Vit E 对照组小鼠上述各指标均无显著变化，DmF 染毒模型组小鼠血清中GOT，GPT 和 XOD 活性及肝组织 mDA 含量均升高（P﹤0.05，P﹤0.01），且肝组织存在明显的病理损伤，肝细胞核固缩，细胞水肿、变性、坏死。与 DmF 染毒模型组比较，DmF+Vit E 干预组小鼠血清 GOT，GPT 和XOD 活性及肝组织 mDA 含量均降低（P﹤0.05，P﹤0.01），肝组织病理损伤明显减轻，几乎看不到变性和水肿细胞。结论 Vit E 对 DmF 引起的小鼠慢性肝损伤具有一定的预防作用。
目的：探討維生素 E（Vit E）對二甲基甲酰胺（DmF）緻小鼠慢性肝損傷的預防作用。方法將40隻健康 SPF 級雄性昆明小鼠隨機分為正常對照組、Vit E 對照組、DmF 染毒模型組和 DmF+Vit E 榦預組，每組10隻。DmF 染毒模型組和 DmF+Vit E 榦預組 ig 給予 DmF 0.5 g·kg-1染毒；2 h 後，Vit E 對照組和 DmF+Vit E 榦預組 ig 給予 Vit E 5 mg·kg-1，每天1次，連續30 d。用試劑盒測定小鼠血清穀草轉氨酶（GOT）、穀丙轉氨酶（GPT）和黃嘌呤氧化酶（XOD）活性及丙二醛（mDA）含量，HE 染色法觀察肝組織病理改變。結果與正常對照組比較，Vit E 對照組小鼠上述各指標均無顯著變化，DmF 染毒模型組小鼠血清中GOT，GPT 和 XOD 活性及肝組織 mDA 含量均升高（P﹤0.05，P﹤0.01），且肝組織存在明顯的病理損傷，肝細胞覈固縮，細胞水腫、變性、壞死。與 DmF 染毒模型組比較，DmF+Vit E 榦預組小鼠血清 GOT，GPT 和XOD 活性及肝組織 mDA 含量均降低（P﹤0.05，P﹤0.01），肝組織病理損傷明顯減輕，幾乎看不到變性和水腫細胞。結論 Vit E 對 DmF 引起的小鼠慢性肝損傷具有一定的預防作用。
목적：탐토유생소 E（Vit E）대이갑기갑선알（DmF）치소서만성간손상적예방작용。방법장40지건강 SPF 급웅성곤명소서수궤분위정상대조조、Vit E 대조조、DmF 염독모형조화 DmF+Vit E 간예조，매조10지。DmF 염독모형조화 DmF+Vit E 간예조 ig 급여 DmF 0.5 g·kg-1염독；2 h 후，Vit E 대조조화 DmF+Vit E 간예조 ig 급여 Vit E 5 mg·kg-1，매천1차，련속30 d。용시제합측정소서혈청곡초전안매（GOT）、곡병전안매（GPT）화황표령양화매（XOD）활성급병이철（mDA）함량，HE 염색법관찰간조직병리개변。결과여정상대조조비교，Vit E 대조조소서상술각지표균무현저변화，DmF 염독모형조소서혈청중GOT，GPT 화 XOD 활성급간조직 mDA 함량균승고（P﹤0.05，P﹤0.01），차간조직존재명현적병리손상，간세포핵고축，세포수종、변성、배사。여 DmF 염독모형조비교，DmF+Vit E 간예조소서혈청 GOT，GPT 화XOD 활성급간조직 mDA 함량균강저（P﹤0.05，P﹤0.01），간조직병리손상명현감경，궤호간불도변성화수종세포。결론 Vit E 대 DmF 인기적소서만성간손상구유일정적예방작용。
OBJECTlVE To study the preventive effect of vitamin E(Vit E)on chronic Iiver injury in-duced by N,N-dimethyIformamide( DmF). METHODS HeaIthy maIe Kunming mice were randomIy divided into 4 groups,10 mice in each group. The mice in DmF modeI group and DmF+Vit E group were ig given DmF 0.5 g·kg-1 ,whiIe Vit E controI group and DmF+Vit E group were ig given Vit E 5 mg·kg-1 after 2 h once a day for 30 d. The activity of serum gIutamic-oxaIoacetic transaminase(GOT),gIutamic-pyruvic transaminase(GPT)and xanthine oxidase(XOD)and the content of maIondiaIdehyde(mDA) in Iiver tissue were measured with corresponding kits. The pathoIogicaI changes of Iiver tissue were ob-served with HE staining. RESULTS Compared with the normaI controI group,the activity of serum GOT,GPT and XOD of the DmF modeI group was significantIy eIevated(P﹤0.05,P﹤0.01),and the Iiver ceII nucIeus pycnosis,ceIIuIar edema,degeneration,necrosis occurred. Compared with DmF modeI group,the activity of serum GOT,GPT and XOD of DmF+Vit E group were aII significantIy reduced(P﹤0.05,P﹤0.01),the content of mDA in Iiver tissue was significantIy diminished(P﹤0.01),but histopatho-IogicaI changes of Iiver tissue were significantIy improved. CONCLUSlON Vit E has preventive effect on chronic Iiver damage induced by DmF through antioxidation.
