CAJ | 학술논문

目的：探讨大鼠感染肺炎链球菌肺炎后血清铁及血红蛋白的变化以及感染程度与血清铁、血红蛋白之间的关系。方法将60只远交群大鼠（SD大鼠）随机分成4组，每组15只，3组为试验组，经口气管插管注入低、中、高3个梯度的菌液（通过菌量梯度反映肺炎感染严重程度），同时设1组未经口气管插管注入菌液对照组，观察第3、5、7天4组大鼠平均血清铁及血红蛋白水平，以及各试验组菌量梯度与血红蛋白及血清铁均值之间的关系。结果中、高菌量组血清铁水平第3天时与对照组相比即显著下降（P＝0．009，P＝0．005），低菌量组第5天时血清铁水平与对照组相比差异有统计学意义（P＝0．007）；中、高菌量组血红蛋白水平第5天时与对照组比较差异有统计学意义（P＝0．031，P＝0．046），低菌量组血红蛋白第3、5、7天与对照组相比差异均无统计学意义（P＞0．05），不同菌量梯度与血清铁含量平均值呈负相关（r＝-0．65、r＝-0．53、r＝-0．61），不同菌量梯度与血红蛋白平均值之间无明确相关性。结论肺炎链球菌感染可导致大鼠血清铁及血红蛋白水平下降，感染严重程度与血清铁水平存在相关性（感染越重，血清铁水平越低），与血红蛋白水平无明确相关性。
목적：탐토대서감염폐염련구균폐염후혈청철급혈홍단백적변화이급감염정도여혈청철、혈홍단백지간적관계。방법장60지원교군대서（SD대서）수궤분성4조，매조15지，3조위시험조，경구기관삽관주입저、중、고3개제도적균액（통과균량제도반영폐염감염엄중정도），동시설1조미경구기관삽관주입균액대조조，관찰제3、5、7천4조대서평균혈청철급혈홍단백수평，이급각시험조균량제도여혈홍단백급혈청철균치지간적관계。결과중、고균량조혈청철수평제3천시여대조조상비즉현저하강（P＝0．009，P＝0．005），저균량조제5천시혈청철수평여대조조상비차이유통계학의의（P＝0．007）；중、고균량조혈홍단백수평제5천시여대조조비교차이유통계학의의（P＝0．031，P＝0．046），저균량조혈홍단백제3、5、7천여대조조상비차이균무통계학의의（P＞0．05），불동균량제도여혈청철함량평균치정부상관（r＝-0．65、r＝-0．53、r＝-0．61），불동균량제도여혈홍단백평균치지간무명학상관성。결론폐염련구균감염가도치대서혈청철급혈홍단백수평하강，감염엄중정도여혈청철수평존재상관성（감염월중，혈청철수평월저），여혈홍단백수평무명학상관성。
Objective To examine the changing levels of serum iron and hemoglobin in Streptococcus pneumoniae-induced rat pneumonia model and the relationship among infection severity,serum iron and hemoglobin.Methods Sixty Sprague Dawley (SD)rats were randomly assigned to one of the four groups,1 5 SD rats in each group,including three treatment groups and one control group. Low, medium and high doses of bacteria were administered to the animals in the treatment groups respectively through tracheal cannula.The severity of pneumonia was indicated by the level of bacterial load.The animals in the control group did not receive bacterial challenge.The mean serum iron and hemoglobin levels were calculated on day 3,5, and 7 to analyze the relationship between bacterial dose and serum iron or hemoglobin levels.Results The serum iron level in the animals receiving medium or high doses of bacteria was significantly lower compared with that in control group on day 3 (P=0.009,P=0.005).The serum iron level in the animals receiving low dose of bacteria showed significant difference compared with that in the control group on day 5 (P=0.007).The hemoglobin level in the animals receiving medium or high doses of bacteria was significantly different from that in the control group on day 5 (P=0.031,P=0.046).The hemoglobin level in the animals receiving low dose of bacteria did not show significant difference compared with that in the control group on day 3,5 or 7.The bacterial dose level was negatively correlated with the mean level of serum iron (correlation coefficient r=-0.65,r=-0.53,r=-0.61,respectively).There was no definite correlation between the bacterial dose and the mean hemoglobin level.Conclusions Streptococcus pneumoniae infection may be associated with lower serum iron and hemoglobin levels in rats.The severity of infection is negatively correlated with serum iron level,but not hemoglobin level.