CAJ | 학술논문

目的：应用白念珠菌全基因组表达谱芯片比较山苍子油处理前后白念珠菌基因的差异性表达，分析山苍子油对白念珠菌整个基因表达谱的影响。方法用山苍子油处理白念珠菌 ATCC90028株90 min，将处理后的菌株命名为白念珠菌ATCC90028-L，分别抽提细胞总 RNA，经杂交、洗涤后，通过扫描分析白念珠菌 ATCC90028株和 ATCC90028-L株全基因组表达谱的差异。结果基因芯片共筛选出491个差异表达基因，与白念珠菌 ATCC90028株比较，在 ATCC90028-L株中有216个基因表达上调，有275个基因表达下调，差异表达的基因数量约占总基因数量的11％（491/4634），差异表达基因主要包括白念珠菌麦角固醇生物合成通路中关键靶酶编码基因（ERGs）、应激反应相关基因、DNA复制与损伤修复相关基因、跨膜分子转运相关基因、能量代谢酶类相关基因等。结论山苍子油可使白念珠菌基因组中约11％基因产生差异性表达，影响较为显著，我们推测山苍子油与唑类抗真菌药物的作用机制相似，均通过对白念珠菌麦角固醇生物合成通路中关键靶酶的影响而起作用，基因芯片筛选出的其他差异表达基因也可能与山苍子油的抗真菌作用机制有关，值得进一步研究。
목적：응용백념주균전기인조표체보심편비교산창자유처리전후백념주균기인적차이성표체，분석산창자유대백념주균정개기인표체보적영향。방법용산창자유처리백념주균 ATCC90028주90 min，장처리후적균주명명위백념주균ATCC90028-L，분별추제세포총 RNA，경잡교、세조후，통과소묘분석백념주균 ATCC90028주화 ATCC90028-L주전기인조표체보적차이。결과기인심편공사선출491개차이표체기인，여백념주균 ATCC90028주비교，재 ATCC90028-L주중유216개기인표체상조，유275개기인표체하조，차이표체적기인수량약점총기인수량적11％（491/4634），차이표체기인주요포괄백념주균맥각고순생물합성통로중관건파매편마기인（ERGs）、응격반응상관기인、DNA복제여손상수복상관기인、과막분자전운상관기인、능량대사매류상관기인등。결론산창자유가사백념주균기인조중약11％기인산생차이성표체，영향교위현저，아문추측산창자유여서류항진균약물적작용궤제상사，균통과대백념주균맥각고순생물합성통로중관건파매적영향이기작용，기인심편사선출적기타차이표체기인야가능여산창자유적항진균작용궤제유관，치득진일보연구。
Objective To analyze the effects of Litsea cubeba oil on gene expression profile of Candida albicans by comparing the differential gene expression profile after exposure to Litsea cubeba oil using genome-wide gene expression array.Methods Candida albicans ATCC90028 was exposed to Litsea cubeba oil for 90 min.Then RNA was isolated and gene expression profiles were compared to identify the differential gene expression profile using cDNA microarray analysis.Results A total of 491 geneswerefoundtoberesponsivetoLitseacubebaoil,accountingforabout11% ofthetotalnumberofgenesinCandida albicans (491/4 634),of which 216 genes were up-regulated and 275 down-regulated.These differentially expressed genes included genes encoding the key target enzyme in ergosterol biosynthesis pathway,genes in stress response,DNA replication and repair,molecular transport,and energy metabolism.Conclusions Litsea cubeba oil has significant effect on the expression of about 1 1% genes of Candida albicans genome.We presume that the genes encoding the key target enzyme in ergosterol biosynthesis pathway may contribute to the action of Litseacubeba oil on Candidaalbicans,which is similar to azole antifungal drugs.However,the role of other differentially expressed genes in the action of Litseacubeba oil on Candidaalbicans remains unclear,which deserves further study to characterize their potential association with the antifungal effect of Litsea cubeba oil.