CAJ | 학술논문

黑李溃疡病为近年来发生在浙江省黑李产区的一种严重病害，主要危害李树当年生、多年生枝和主干，引起枝条枯死和整株死亡，继而毁灭整个黑李果园。通过对黑李溃疡病典型症状样本的采集、病原菌的分离、纯化和致病性测定，明确引起该病的病原菌为一种细菌。通过对黑李溃疡病菌的菌体形态和培养特性观察，生理生化反应、脂肪酸分析、16 S rDNA序列分析和种的特异性引物的检测分析，证明该病原细菌为树生黄单胞菌李变种Xanthomonas arboricola pv．pruni（Smith）Vauterin et al．。
흑리궤양병위근년래발생재절강성흑리산구적일충엄중병해，주요위해리수당년생、다년생지화주간，인기지조고사화정주사망，계이훼멸정개흑리과완。통과대흑리궤양병전형증상양본적채집、병원균적분리、순화화치병성측정，명학인기해병적병원균위일충세균。통과대흑리궤양병균적균체형태화배양특성관찰，생리생화반응、지방산분석、16 S rDNA서렬분석화충적특이성인물적검측분석，증명해병원세균위수생황단포균리변충Xanthomonas arboricola pv．pruni（Smith）Vauterin et al．。
Black plum ( Prunus americana) canker is a serious disease occurring in recent years in black plum plantation in Zhejiang Province .It mainly infects current-year shoots , perennial branches and trunks , causing the branches withered and the whole plant died , and finally destroyed the whole black plum orchard .In this study, we collected the typical symptom samples of this disease , isolated and purified the pathogen , and then the pathogenicity determination results showed that the pathogen causing black pulm canker was a kind of bacteria .The pathogenic bacteria was identified as Xanthomonas arboricola pv.Pruni(smith) Vauterin et al.based on the bacteria cell morphology , cultural characteristics , physiological and biochemical characteristics , analysis of fatty acid methylesters (FAME) and 16S rDNA gene sequence.