中国感染与化疗杂志
中國感染與化療雜誌
중국감염여화료잡지
CHINESE JOURNAL OF INFECTION AND CHEMOTHERAPY
2014年
4期
286-290
,共5页
肠杆菌科%喹诺酮类%质粒%耐药性%细菌%I类整合子
腸桿菌科%喹諾酮類%質粒%耐藥性%細菌%I類整閤子
장간균과%규낙동류%질립%내약성%세균%I류정합자
Enterobacteriaceae%quinolone%plasmid%drug resistance%class l integron
目的：调查肠杆菌科细菌中质粒介导喹诺酮类耐药基因（PMQR基因）的现状、基因型以及 PMQR基因阳性菌株携带Ⅰ类整合子的情况及其相关性。方法 PCR法对125株肠杆菌科细菌（80株大肠埃希菌、18株肺炎克雷伯菌和27株阴沟肠杆菌）进行PMQR基因和Ⅰ类整合子检测。对阳性扩增产物进行测序分析并对阳性菌株做接合试验，采用琼脂倍比稀释法测定供体菌、受体菌和接合子对喹诺酮类和其他抗菌药物的 MIC。结果125株肠杆菌科细菌检出16株（12．8％）qnr基因阳性，其中8株携带qnrS1基因，8株携带qnrB6基因；另检出15株（12．0％）携带aac（6′）-Ib-cr基因。20株PMQR基因阳性菌株携带Ⅰ类整合子。26株PMQR基因阳性的菌株中有12株接合成功，典型Ⅰ类整合子阳性的菌株中有7株接合成功。与受体菌相比，喹诺酮类等抗菌药物对接合子的 MIC值均有不同程度的提高。结论肠杆菌科细菌中存在 PMQR基因的流行，PMQR阳性菌株可同时携带整合子基因，这些耐药基因均具有水平转移的特性，故应引起高度重视。
目的：調查腸桿菌科細菌中質粒介導喹諾酮類耐藥基因（PMQR基因）的現狀、基因型以及 PMQR基因暘性菌株攜帶Ⅰ類整閤子的情況及其相關性。方法 PCR法對125株腸桿菌科細菌（80株大腸埃希菌、18株肺炎剋雷伯菌和27株陰溝腸桿菌）進行PMQR基因和Ⅰ類整閤子檢測。對暘性擴增產物進行測序分析併對暘性菌株做接閤試驗，採用瓊脂倍比稀釋法測定供體菌、受體菌和接閤子對喹諾酮類和其他抗菌藥物的 MIC。結果125株腸桿菌科細菌檢齣16株（12．8％）qnr基因暘性，其中8株攜帶qnrS1基因，8株攜帶qnrB6基因；另檢齣15株（12．0％）攜帶aac（6′）-Ib-cr基因。20株PMQR基因暘性菌株攜帶Ⅰ類整閤子。26株PMQR基因暘性的菌株中有12株接閤成功，典型Ⅰ類整閤子暘性的菌株中有7株接閤成功。與受體菌相比，喹諾酮類等抗菌藥物對接閤子的 MIC值均有不同程度的提高。結論腸桿菌科細菌中存在 PMQR基因的流行，PMQR暘性菌株可同時攜帶整閤子基因，這些耐藥基因均具有水平轉移的特性，故應引起高度重視。
목적：조사장간균과세균중질립개도규낙동류내약기인（PMQR기인）적현상、기인형이급 PMQR기인양성균주휴대Ⅰ류정합자적정황급기상관성。방법 PCR법대125주장간균과세균（80주대장애희균、18주폐염극뢰백균화27주음구장간균）진행PMQR기인화Ⅰ류정합자검측。대양성확증산물진행측서분석병대양성균주주접합시험，채용경지배비희석법측정공체균、수체균화접합자대규낙동류화기타항균약물적 MIC。결과125주장간균과세균검출16주（12．8％）qnr기인양성，기중8주휴대qnrS1기인，8주휴대qnrB6기인；령검출15주（12．0％）휴대aac（6′）-Ib-cr기인。20주PMQR기인양성균주휴대Ⅰ류정합자。26주PMQR기인양성적균주중유12주접합성공，전형Ⅰ류정합자양성적균주중유7주접합성공。여수체균상비，규낙동류등항균약물대접합자적 MIC치균유불동정도적제고。결론장간균과세균중존재 PMQR기인적류행，PMQR양성균주가동시휴대정합자기인，저사내약기인균구유수평전이적특성，고응인기고도중시。
Objective To explore the distribution and genotypes of plasmid-mediated quionlone resistance (PMQR)genes and intI1 integrase genes in Enterobacteriaceae isolates.Methods The PMQR genes and intI1 integrase genes were identified by polymerase chain reaction in the nonduplicate strains of E.coli (80),E.cloacae (18)and K.pneunoniae (27).The positive PCR products were subj ect to DNA sequencing analysis.The gene-positive strains were tested by conj ugation experiment.The minimum inhibitory concentrations (MICs)of donor,recipient strains and transconj ugants were tested by agar dilution method with quinolones and other antimicrobial agents.Results Sixteen (12.8%)of the 125 Enterobacteriaceae isolates were qnr gene positive,including 8 qnrS1 positive and 8 qnrB6 positive.Furthermore,the aac(6′)-Ib-cr gene was identified in 15 (12.0%) strains.Twenty PMQR-positive isolates harbored intI1 integrase gene.The conjugation experiments were successful in 12 of the 26 PMQR-positive isolates and 7 of the 20 intI1-positive isolates.The MICs of quinolones and other antimicrobial agents against the transconj ugants were higher than the MIC values against recipient strains.Conclusions The PMQR genes are prevalent in Enterobacteriaceae isolates.The PMQR-positive isolates can co-harbor integrase genes.These resistance genes have the feature of horizontal transfer,to which close attention should be paid.
