CAJ | 학술논문

巨囊泡作为细胞的简化模型，其相分离与出芽动力学规律已引起许多领域科学家的关注。本实验采用DPPC/DOPC/Chol的三组分形成的巨囊泡作为模型，借助荧光显微镜观察该三组分体系侧向分离的相结构图，并对微畴的成长过程作了系统的观察研究和理论分析。实验发现：从高温的均相区域淬灭到低温的分相区域，膜表面发生侧向分离形成微畴。体系内胆固醇的掺入量的多少会影响磷脂膜的相结构和膜内微畴的成长，固定 DOPC/DPPC为1：1的前提下，微畴尺寸随着胆固醇掺入量的增加而变大。
거낭포작위세포적간화모형，기상분리여출아동역학규률이인기허다영역과학가적관주。본실험채용DPPC/DOPC/Chol적삼조분형성적거낭포작위모형，차조형광현미경관찰해삼조분체계측향분리적상결구도，병대미주적성장과정작료계통적관찰연구화이론분석。실험발현：종고온적균상구역쉬멸도저온적분상구역，막표면발생측향분리형성미주。체계내담고순적참입량적다소회영향린지막적상결구화막내미주적성장，고정 DOPC/DPPC위1：1적전제하，미주척촌수착담고순참입량적증가이변대。
Giant Unilamellar Vesicles (GUVs)as a simplified model of cell,its kinetics of phase separa-tion and budding have attracted many scientists attention.In this paper,the typical membrane system which consisted of DOPC/DPPC/Chol was used,the experiment studies and theoretically analyzes the growth process of domains by observing the structure diagram of its lateral separation with the help of fluorescence microscopy.It showed that:domains were forming through lateral separation on the sur-face of membrane from the homogeneous region at high temperature quenching to the heterogeneous re-gion at low temperature.Different component of Cholesterol would influence the phase structure of phospholipids‘membrane and the growth of domains within the membrane,with varying cholesterol composition against a fixed 1∶1 ratio of DOPC/DPPC,the domain radius increasing with the cholesterol composition.