CAJ | 학술논문

为了监测果蔬中植物激素的污染状况，本研究首先利用荧光偏振信号法计算荧光偏振度和荧光各向异性度来区分激动素和6-苄基腺嘌呤2种细胞分裂素，然后建立了同时测定果蔬中激动素和6-卞基腺嘌呤残留量的L-苯丙氨酸荧光猝灭法。结果显示：L-苯丙氨酸-激动素体系对应着较大的荧光各向异性度(荧光偏振度)，而L-苯丙氨酸-6-苄基腺嘌呤体系则对应着较小的荧光各向异性度(荧光偏振度)。激动素和6-苄基腺嘌呤的回收率均为95.00%~104.00%，相对标准偏差为2.22%~4.13%。结果表明该方法可用于市售果蔬李子和番茄样品表皮中激动素和6-苄基腺嘌呤的测定。
위료감측과소중식물격소적오염상황，본연구수선이용형광편진신호법계산형광편진도화형광각향이성도래구분격동소화6-변기선표령2충세포분렬소，연후건립료동시측정과소중격동소화6-변기선표령잔류량적L-분병안산형광졸멸법。결과현시：L-분병안산-격동소체계대응착교대적형광각향이성도(형광편진도)，이L-분병안산-6-변기선표령체계칙대응착교소적형광각향이성도(형광편진도)。격동소화6-변기선표령적회수솔균위95.00%~104.00%，상대표준편차위2.22%~4.13%。결과표명해방법가용우시수과소리자화번가양품표피중격동소화6-변기선표령적측정。
To monitor the pollution of plant hormones in frutis and vegetables, the degree of fluorescence polarization and the degree of fluorescence anisotropy were employed based on the measurements of the polarized fluorescence signals to distinguish two natural exogenous cytokinins of kinetin ( KT) and 6-benzylaminourine firstly. Then a method of simultaneous determination of KT and 6-benzylaminourine was established by L-Phe fluorescence quenching. L-Phe-KT system exhibited a large degree of fluorescence anisotropy ( fluorescence polarization) , while the L-Phe-6-BA system showed a small degree. The recoveries of KT and 6-BA were both 95. 00%-104. 00%,with the relative standard deviation(RSD) of 2. 22%-4. 13%. The method is applicable to simultaneous determination of kinetin and 6-benzylaminourine in plum skin and tomato skin.