CAJ | 학술논문

为了探索亚硒酸钠对小鼠胚胎干细胞和延边奶山羊-鼠异种重构胚抗氧化损伤的保护能力,以及亚硒酸钠提高小鼠胚胎干细胞和延边奶山羊-鼠异种重构胚发育率的作用,通过MTT[3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐]法检测在亚硒酸钠处理下,小鼠胚胎干细胞和奶山羊-鼠异种重构胚抗氧化损伤的增殖能力,并统计重构胚和异种重构胚的发育率。结果显示,经3.5μmol/L亚硒酸钠处理的90.0μmol/L H2 O2氧化损伤的小鼠胚胎干细胞的增殖率显著提高。亚硒酸钠处理的小鼠胚胎干细胞重构胚和延边奶山羊-鼠胎儿皮肤成纤维细胞异种重构胚的卵裂率显著高于未经亚硒酸钠处理的胚胎干细胞。表明亚硒酸钠可提高小鼠胚胎干细胞重构胚和延边奶山羊-鼠胎儿皮肤成纤维细胞异种重构胚的发育率。
위료탐색아서산납대소서배태간세포화연변내산양-서이충중구배항양화손상적보호능력,이급아서산납제고소서배태간세포화연변내산양-서이충중구배발육솔적작용,통과MTT[3-(4,5-이갑기새서-2)-2,5-이분기사담서추염]법검측재아서산납처리하,소서배태간세포화내산양-서이충중구배항양화손상적증식능력,병통계중구배화이충중구배적발육솔。결과현시,경3.5μmol/L아서산납처리적90.0μmol/L H2 O2양화손상적소서배태간세포적증식솔현저제고。아서산납처리적소서배태간세포중구배화연변내산양-서태인피부성섬유세포이충중구배적란렬솔현저고우미경아서산납처리적배태간세포。표명아서산납가제고소서배태간세포중구배화연변내산양-서태인피부성섬유세포이충중구배적발육솔。
To define the protection of antioxidation damage on embryonic stem cells and goat-mouse interspecies re-constructed embryos by sodium selenite, and the effect of development rates of mouse embryonic stem cells and goat-mouse interspecies reconstructed embryo improved by sodium selerite, the proliferation capability of antioxidation damage of em-bryonic stem cells from mouse and goat-mouse interspecies reconstructed embryos were detected by the method of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT], and the development rates of reconstructed embryos and interspecies reconstructed embryos were recorded. The proliferation rate of embryonic stem cells damaged by 90. 0 μmol/L H2 O2 was significantly higher in 3. 5μmol/L sodium selenite group than those in other groups. The cleavage rates of recon-structed embryo from mouse embryonic stem cells and goat-mouse interspecies neconstructed embryos treated by sodium sel-enite were significantly higher than those without sodium selenite. In sum, sodium selenite could improve the developmental rates of reconstructed embryos from mouse embryonic stem cells and goat-mouse interspecies recon-structed embryos.