CAJ | 학술논문

苏钟猪是江苏省农业科学院利用太湖猪为母本,长白猪为父本育成的品种。为苏钟猪个体身份识别及猪肉产品溯源之需,对苏钟猪个体身份 SNP识别进行研究。本研究共设计29对引物,并从中筛选7对引物用于苏钟猪的个体身份识别。7对引物扩增产物能直接测序且测序图谱背景干净,序列阅读无歧义。7对引物共扩增52个SNP位点,经关联性分析及杂合度过滤(H≥0.1)后,选留41个 SNP位点用于苏钟猪个体身份识别的数字条形码编制；同时,编制了7头公猪和12头母猪所生的96头苏钟猪个体身份 SNP识别的数字条形码,数字条形码很好地区分了96头苏钟猪的个体身份。根据96头苏钟猪条形码编制时分离到的各引物对扩增片段的基因型数,7对引物扩增的41个 SNP位点,理论上可用于近5.00×106头猪的个体身份识别。因此,本研究建立的苏钟猪个体身份SNP识别的数字条形码编制方法,可用于规模猪场苏钟猪的个体身份识别及肉产品溯源。
소종저시강소성농업과학원이용태호저위모본,장백저위부본육성적품충。위소종저개체신빈식별급저육산품소원지수,대소종저개체신빈 SNP식별진행연구。본연구공설계29대인물,병종중사선7대인물용우소종저적개체신빈식별。7대인물확증산물능직접측서차측서도보배경간정,서렬열독무기의。7대인물공확증52개SNP위점,경관련성분석급잡합도과려(H≥0.1)후,선류41개 SNP위점용우소종저개체신빈식별적수자조형마편제；동시,편제료7두공저화12두모저소생적96두소종저개체신빈 SNP식별적수자조형마,수자조형마흔호지구분료96두소종저적개체신빈。근거96두소종저조형마편제시분리도적각인물대확증편단적기인형수,7대인물확증적41개 SNP위점,이론상가용우근5.00×106두저적개체신빈식별。인차,본연구건립적소종저개체신빈SNP식별적수자조형마편제방법,가용우규모저장소종저적개체신빈식별급육산품소원。
Suzhong swine is a hybrid breed derived from Taihu sows and Landrace boars. To identify individual Suzhong swine and trace the source of pork product, 52 single nucleotide polymorphisms ( SNP) were developed in the Suzhong swine genomic DNA fragments using 7 pairs of primers out of 29. Among the SNPs, 41 with heterozygosities more than 0. 1 were selected for Suzhong pigs swine genotyping. By converting the genetic loci with sequences of AA, TT, GG, CC, AT, AG, AC, TG, TC, and GC into digital number of 0, 1, 2, 3, 4, 5, 6, 7, 8, and 9, respective-ly, 96 digital barcodes, unique for each individual pig produced, were generated for 96 Suzhong swine derived from 7 boars and 12 sows. Theoretically, the barcodes based on 41 SNPs amplified by the 7 primer pairs can be used for distinguishing about 5 000 000 individual pigs.