CAJ | 학술논문

以金星无核葡萄叶片为试验材料,采用RT-PCR结合RACE技术克隆得到IPK2同源基因全长cDNA序列,命名为VvIPK2(GenBank登录号：KF752483),全长1853 bp,含有1个918 bp的开放阅读框,编码305个氨基酸,预测VvIPK2蛋白质分子量为3.421×104,理论等电点为5.38。系统进化分析表明,VvIPK2编码的氨基酸序列与拟南芥、盐芥、大豆和菜豆的一致性分别为65.23%、63.56%、54.84%和52.18%。荧光定量RT-PCR分析结果表明,VvIPK2在葡萄叶片和茎中的表达水平高于根和花,葡萄霜霉病菌侵染后72 h内均可诱导VvIPK2基因的表达,且相对表达量均高于对照(处理0 h),说明VvIPK2基因在葡萄应答霜霉病菌侵染过程中可能发挥着重要作用。
이금성무핵포도협편위시험재료,채용RT-PCR결합RACE기술극륭득도IPK2동원기인전장cDNA서렬,명명위VvIPK2(GenBank등록호：KF752483),전장1853 bp,함유1개918 bp적개방열독광,편마305개안기산,예측VvIPK2단백질분자량위3.421×104,이론등전점위5.38。계통진화분석표명,VvIPK2편마적안기산서렬여의남개、염개、대두화채두적일치성분별위65.23%、63.56%、54.84%화52.18%。형광정량RT-PCR분석결과표명,VvIPK2재포도협편화경중적표체수평고우근화화,포도상매병균침염후72 h내균가유도VvIPK2기인적표체,차상대표체량균고우대조(처리0 h),설명VvIPK2기인재포도응답상매병균침염과정중가능발휘착중요작용。
A full length of IPK2 gene related to downy mildew resistance was isolated from grapevine Venus Seedless by RT-PCR and RACE method and designated VvIPK2 ( GenBank accession No. KF752483 ) . The cDNA of VvIPK2 is 1 853 bp in length and contains an open reading frame of 918 bp. The predicted VvIPK2 protein has 305 amino acids with an estimated molecular mass of 3. 421×104 and an isoelectric point of 5. 38. Phylogenetic analysis shows that VvIPK2 shares 65. 23%, 63. 56%, 54. 84% and 52. 18% similarities with IPK2 in Arabidopsis thaliana, Thellungiella halophila, Glycine max and Phaseolus vulgaris. qRT-PCR analysis reveals that the expressions of VvIPK2 gene in leaf and stem are higher than those in root and flower. The expression of VvIPK2 gene was up-regulated in 72 h after the inoculation of Plasmopara vitico-la, and the level is much higher than that of the control ( treated for 0 h) . It is suggested that VvIPK2 gene may play an im-portant role in response to grapevine downy mildew.