CAJ | 학술논문

本研究通过农杆菌介导法将拟南芥耐盐基因AtCHX23导入玉米自交系郑58中,用PCR、Southern blot和RT-PCR法对转化玉米进行检测,并在150 mmol/L 盐( NaCl)胁迫下对T3代转基因玉米和野生型进行耐盐性分析。结果显示：共获得26株转基因阳性植株；挑选长势较好的2个 PCR阳性株系进行 Southern blot鉴定,确定AtCHX23基因以单拷贝的形式成功插入到玉米基因组中,且AtCHX23基因在转基因玉米中过量表达。非盐胁迫条件下,野生型和2个转基因株系间生长状态及其可溶性糖、脯氨酸和丙二醛含量无显著差异；在150 mmol/L 盐处理下,2个转基因株系的生长状态优于野生型,2个转基因玉米株系的可溶性糖和脯氨酸含量均高于野生型,丙二醛的含量低于野生型。综上所述：AtCHX23基因过量表达可以提高玉米苗期的耐盐性。
본연구통과농간균개도법장의남개내염기인AtCHX23도입옥미자교계정58중,용PCR、Southern blot화RT-PCR법대전화옥미진행검측,병재150 mmol/L 염( NaCl)협박하대T3대전기인옥미화야생형진행내염성분석。결과현시：공획득26주전기인양성식주；도선장세교호적2개 PCR양성주계진행 Southern blot감정,학정AtCHX23기인이단고패적형식성공삽입도옥미기인조중,차AtCHX23기인재전기인옥미중과량표체。비염협박조건하,야생형화2개전기인주계간생장상태급기가용성당、포안산화병이철함량무현저차이；재150 mmol/L 염처리하,2개전기인주계적생장상태우우야생형,2개전기인옥미주계적가용성당화포안산함량균고우야생형,병이철적함량저우야생형。종상소술：AtCHX23기인과량표체가이제고옥미묘기적내염성。
AtCHX23, a putative Na+(k+)/H+antiporter on the chloroplast envelope, was transferred into maize in-bred line Zheng 58 by Agrobacterium tumefaciens-mediated transformation. PCR results showed that 26 transgenic plants with AtCHX23 gene were obtained, and two lines of which were verified as insertion of single gene by Southern blot. RT-PCR analyses showed that AtCHX23 was over-expressed in transgenic lines. Under no salt stress, the growth and the con-tents of soluble sugar( WSS) , proline and methane dicarboxylic aldehyde ( MDA) contents did not differ between wild type and two transgenic lines. However, the transgenic plants under the stress of 150 mmol/L NaCl at seedling stages grew bet-ter than wild type did, and the WSS and proline contents of transgenic lines were higher than that of wild type; however, the MDA contents were lower than that of wild type. These results demonstrated that over-expression of AtCHX23 signifi-cantly improved the salt tolerance of maize seedling.