CAJ | 학술논문

为了探究转玉米C4双基因水稻抗旱性,以野生型恢复系水稻R299及其转PEPC&PPDK基因水稻恢复系纯合品种R2P为材料,木村B营养液培养至3叶1心。对两种水稻在20%浓度(质量体积比)PEG6000(聚乙二醇)模拟干旱条件下光合及相关生理生化指标的变化进行了比较。结果表明：在正常木村B营养液水培条件下,转基因株系R2P的叶绿体超微结构、光合及其相关生理指标与R299相比,未见明显差异；但在20% PEG6000模拟干旱条件下,两种水稻之间显示出了明显的变化,表现为 R2P的光合速率、C4光合酶活性、SOD(超氧化物歧化酶)活性、Pro(游离脯氨酸)含量均显著高于R299,叶绿体超微结构较R299稳定,而MDA(丙二醛)含量、O2·-(超氧阴离子)产生速率显著低于R299。说明转C4双基因水稻抗旱性增强,光合及抗氧化系统在PEG6000模拟干旱条件下能保持相对较高的稳定性。
위료탐구전옥미C4쌍기인수도항한성,이야생형회복계수도R299급기전PEPC&PPDK기인수도회복계순합품충R2P위재료,목촌B영양액배양지3협1심。대량충수도재20%농도(질량체적비)PEG6000(취을이순)모의간한조건하광합급상관생리생화지표적변화진행료비교。결과표명：재정상목촌B영양액수배조건하,전기인주계R2P적협록체초미결구、광합급기상관생리지표여R299상비,미견명현차이；단재20% PEG6000모의간한조건하,량충수도지간현시출료명현적변화,표현위 R2P적광합속솔、C4광합매활성、SOD(초양화물기화매)활성、Pro(유리포안산)함량균현저고우R299,협록체초미결구교R299은정,이MDA(병이철)함량、O2·-(초양음리자)산생속솔현저저우R299。설명전C4쌍기인수도항한성증강,광합급항양화계통재PEG6000모의간한조건하능보지상대교고적은정성。
The drought tolerance of transgenic rice with maize PEPC & PPDK genes were studied using wild-type rice restorer line R299 and its PEPC & PPDK transgenic rice homozygous restorer line R2 P as materi-als, cultured in Kimura B nutrient solution to three-leaf period. The photosynthesis, related physiological and biochemical indexes of R2P and R299 subjected to PEG6000 simulated drought stress were compared. The chloroplast ultramicrostructure, photosynthesis and the related antioxidant physiological indexes had no significant difference between R2P and R299 cultured in Kimura B nutrient solution. However, under drought stress, R2P showed faster photosynthesis rate, higher activities of C4 photosynthetic enzyme and SOD ( superoxide dismutase) , higher content of proline, and greater stability of chloroplast ultrastructure than R299, and lower the content of MDA and generation rate of superoxide anion ( O2·-) . The results indicated that the PEPE and PPDK transgenic rice R2 P was more tolerant to the drought.