CAJ | 학술논문

目的：建立能够实现甲基化敏感扩增多态性（MSAP）荧光检测初始数据自动分析的方法。方法：以17周龄的北京油鸡为实验材料，应用荧光标记的MSAP方法对肌肉组织基因组DNA甲基化水平进行检测，对获得的MSAP荧光检测初始数据通过直接取整法、整区间放置法和半区间放置法等3种计算机处理方法进行分析和比较。结果：对于MSAP荧光检测初始数据的自动转换，半区间放置法与标准的人工转换方法最接近（P>0.05），整区间放置法次之（P>0.05），而直接取整法与标准的人工转换方法差异最大，达到显著水平（P<0.05）。结论：整区间放置法和半区间放置法适用于MSAP荧光检测初始数据的自动分析，建立了基于半区间放置法的在线自动分析软件MSAP Analyst，可实现MSAP荧光检测初始数据的自动分析。
목적：건립능구실현갑기화민감확증다태성（MSAP）형광검측초시수거자동분석적방법。방법：이17주령적북경유계위실험재료，응용형광표기적MSAP방법대기육조직기인조DNA갑기화수평진행검측，대획득적MSAP형광검측초시수거통과직접취정법、정구간방치법화반구간방치법등3충계산궤처리방법진행분석화비교。결과：대우MSAP형광검측초시수거적자동전환，반구간방치법여표준적인공전환방법최접근（P>0.05），정구간방치법차지（P>0.05），이직접취정법여표준적인공전환방법차이최대，체도현저수평（P<0.05）。결론：정구간방치법화반구간방치법괄용우MSAP형광검측초시수거적자동분석，건립료기우반구간방치법적재선자동분석연건MSAP Analyst，가실현MSAP형광검측초시수거적자동분석。
Objective: To built a suitable automatic analysis system based on compute to process the initial da-ta of methylation level detected by fluorescence labeled methylation sensitive amplified polymorphism(MSAP). Methods: With fluorescently labeled MSAP, the DNA methylation levels in muscle tissues from 17-week-old Bei-jing You chicken were detected. The initial data of MSAP were analyzed using manual processing usually as a standard solution, and three automatic processing systems based on compute: direct integral method, whole interval method and half interval method. Results: In the analysis of initial data of methylation level detected by fluores-cence labeled MSAP, no differences was found between manual processing and whole interval method(P>0.05) or half interval method(P>0.05), and the half interval method is more near to manual processing than whole interval method, but significant difference appeared between manual processing and direct integral method(P<0.05). Conclu-sion: The whole interval and half interval methods as a substitute for manual processing can be used to realize the automatic analysis of initial data of methylation level detected by fluorescence labeled MSAP, and one new on-line software named MSAP Analyst based on half interval method was built.