CAJ | 학술논문

目的：为了研究c-Myb蛋白各结构域的功能，构建c-Myb的6种截短突变体c-Myb-1~c-Myb-6的真核表达载体，测定不同突变体对c-Myc蛋白表达的影响。方法：以野生型c-Myb为模板，PCR扩增c-Myb-1~c-Myb-6片段，分别克隆到pcDNA3.0-FLAG载体，Western印迹检测克隆载体在293T细胞内的表达；将上述载体与野生型c-Myb转染乳腺癌细胞株MCF-7，检测其对c-Myb下游基因c-Myc表达的影响。结果：构建了c-Myb-1~c-Myb-6表达载体，与野生型c-Myb相比，c-Myb-1、c-Myb-4、c-Myb-5均能够促进MCF-7细胞中c-Myc的表达，而c-Myb-2、c-Myb-3、c-Myb-6则不能。结论：该研究为进一步探讨c-Myb在乳腺癌中的功能奠定了基础。
목적：위료연구c-Myb단백각결구역적공능，구건c-Myb적6충절단돌변체c-Myb-1~c-Myb-6적진핵표체재체，측정불동돌변체대c-Myc단백표체적영향。방법：이야생형c-Myb위모판，PCR확증c-Myb-1~c-Myb-6편단，분별극륭도pcDNA3.0-FLAG재체，Western인적검측극륭재체재293T세포내적표체；장상술재체여야생형c-Myb전염유선암세포주MCF-7，검측기대c-Myb하유기인c-Myc표체적영향。결과：구건료c-Myb-1~c-Myb-6표체재체，여야생형c-Myb상비，c-Myb-1、c-Myb-4、c-Myb-5균능구촉진MCF-7세포중c-Myc적표체，이c-Myb-2、c-Myb-3、c-Myb-6칙불능。결론：해연구위진일보탐토c-Myb재유선암중적공능전정료기출。
Objective: To construct eukaryotic expression vectors containing six deletion mutants c-Myb-1~c-Myb-6 and to detect the impact of different isoforms on the the expression of c-Myc. Methods: c-Myb-1~c-Myb-6 genes were amplified from wild type of c-Myb using PCR and inserted into the vector pcNDA3.0-FLAG. West-ern blot analysis was used to detect the expression of these isoforms. Transfected these vectors and c-Myb into MCF-7 cells and detected the impact on the expression of c-Myc which regulated by c-Myb. Results: c-Myb-1~c-Myb-6 were successfully constructed. Same as wild type c-Myb, c-Myb-1, c-Myb-4 and c-Myb-5 could en-hance the expression of c-Myc in MCF-7, but c-Myb-2, c-Myb-3 and c-Myb-6 could not. Conclusion: This study could be a basis to further detect the function of c-Myb in breast cancer.