CAJ | 학술논문

目的：研究 HBsAg阳性肝癌中PT EN基因的表达，分析PT EN蛋白表达与肝癌病理类型、分化程度的关系。方法采集2009年-2011年在滨州医学院附属医院住院治疗的 HBsAg阳性的原发性肝癌29例患者血液及术后新鲜肝组织，通过RT-PCR扩增PT EN基因，采用免疫组织化学法检测羊抗人PT EN多克隆抗体。结果 PT EN基因位于254 bp处PT EN基因扩增条带及380 bp处GAPDH内参基因条带。肝癌组织中PTEN阳性检出率为69.0％（20/29），相对表达量为0.713±0.125。PT EN蛋白免疫组化表达结果显示PT EN阳性染色呈棕黄色颗粒状或片状，均定位于细胞质中。在表达程度上，肝癌组织PT EN蛋白表达（＋）为4例，（＋＋）为13例，（＋＋＋）为5例。肝细胞癌组与胆管细胞癌组的PT EN蛋白强阳性表达例数分别为16例、2例，两组存在差距（χ2＝4．398，P＜0.05），即病理类型与 PT EN蛋白表达有关；高分化组与低分化组的PTEN蛋白强阳性表达例数分别为13例、5例，高分化组PTEN蛋白表达较低分化组高（χ2＝5.578，P＜0.05），即肝癌分化程度与PT EN蛋白表达有关。结论肝癌组织中PT EN基因高表达，与其PT EN基因表达基本相符。
목적：연구 HBsAg양성간암중PT EN기인적표체，분석PT EN단백표체여간암병리류형、분화정도적관계。방법채집2009년-2011년재빈주의학원부속의원주원치료적 HBsAg양성적원발성간암29례환자혈액급술후신선간조직，통과RT-PCR확증PT EN기인，채용면역조직화학법검측양항인PT EN다극륭항체。결과 PT EN기인위우254 bp처PT EN기인확증조대급380 bp처GAPDH내삼기인조대。간암조직중PTEN양성검출솔위69.0％（20/29），상대표체량위0.713±0.125。PT EN단백면역조화표체결과현시PT EN양성염색정종황색과립상혹편상，균정위우세포질중。재표체정도상，간암조직PT EN단백표체（＋）위4례，（＋＋）위13례，（＋＋＋）위5례。간세포암조여담관세포암조적PT EN단백강양성표체례수분별위16례、2례，량조존재차거（χ2＝4．398，P＜0.05），즉병리류형여 PT EN단백표체유관；고분화조여저분화조적PTEN단백강양성표체례수분별위13례、5례，고분화조PTEN단백표체교저분화조고（χ2＝5.578，P＜0.05），즉간암분화정도여PT EN단백표체유관。결론간암조직중PT EN기인고표체，여기PT EN기인표체기본상부。
Objective To research the association of Primary liver cancer (PLC) with HBsAg positive and PTEN gene ,analy-sis the relationship between PTEN protein expression and pathological type and differentiation degree .Methods The cases of blood and postoperative fresh organization with chronic HBV infection were selected from the inpatient of Binzhou medical uni-versity affiliated hospital among 2009 to 2011 .The PTEN genes were amplificated by RT-PCR ,the immunohistochemical tes-ting of antiviral sheep PTEN polyclonal antibody were chosen .Results PCR amplification of PTEN gene which located in 254 bp PTEN gene amplified bands and 380 bp GAPDH reference gene bands were got in liver cancer tissue .The PTEN-positive de-tection rate was 69.0% (20/29) in foci organization ,the relative expression was 0.713 ± 0.125 .PTEN protein expression im-munohistochemistry results showed that the PTEN-positive staining was located in the cytoplasm ,showing brown yellow granu-lar or flake .In the expression level ,the (+ ) expression of PTEN protein was 4 cases ,the (++ ) expression of PTEN protein was 13 cases ,the (+++ ) expression of PTEN protein was 5 cases .The number of strong PTEN protein expression in hepatic cellular cancer group and cholangiohepatoma group was respectively 16 and 2 ,differences between the two groups was statisti-cally significant (χ2 =4.398 ,P<0.05) ,namely pathologic types and PTEN protein expression had correlation .The number of high PTEN protein expression in high-differentiated group and low-differentiation group was respectively 13 and 5 .Differences between the two groups was statistically significant (χ2 =5.578 , P<0.05) ,namely differentiation degree of liver cancer had correlation with PTEN protein expression .Conclusions PTEN gene were highly expressed in foci organization ,which was con-sistent with those PTEN gene .