CAJ | 학술논문

目的：观察罗格列酮（ RGZ）对腹膜透析相关性腹膜纤维化中TGF-β1和CTGF的表达的影响。方法：36只SD大鼠随机分为4组：空白对照组、模型组、低浓度RGZ组、高浓度RGZ组（每组n＝9）。除空白对照组外其余大鼠均置入腹透管，除空白组外其余组均采用高糖腹膜透析液＋红霉素制作慢性腹膜透析腹膜纤维化模型。低浓度RGZ组、高浓度RGZ组分别采用1．5 mg/kg RGZ、15 mg/kg RGZ治疗。透析8周后处死大鼠，取壁层腹膜行H-E及Masson染色，观察大鼠腹膜形态；免疫组化检测腹膜转化生长因子（ TGF-β1）和结缔组织生长因子（ CTGF）的表达情况。结果：与空白对照组相比，模型组间皮下基质增厚，血管增生及炎症细胞浸润明显增加（ P﹤0．05）；TGF-β1、CTGF表达水平明显增高（ P﹤0．05）；RGZ能改善上述病理改变，下调TGF-β1、CTGF表达（P﹤0．05）；两种剂量间差异无统计学意义（P﹥0．05）。结论：在大鼠腹膜透析相关性腹膜纤维化模型中加用罗格列酮能有效抑制TGF-β1及CTGF表达。
목적：관찰라격렬동（ RGZ）대복막투석상관성복막섬유화중TGF-β1화CTGF적표체적영향。방법：36지SD대서수궤분위4조：공백대조조、모형조、저농도RGZ조、고농도RGZ조（매조n＝9）。제공백대조조외기여대서균치입복투관，제공백조외기여조균채용고당복막투석액＋홍매소제작만성복막투석복막섬유화모형。저농도RGZ조、고농도RGZ조분별채용1．5 mg/kg RGZ、15 mg/kg RGZ치료。투석8주후처사대서，취벽층복막행H-E급Masson염색，관찰대서복막형태；면역조화검측복막전화생장인자（ TGF-β1）화결체조직생장인자（ CTGF）적표체정황。결과：여공백대조조상비，모형조간피하기질증후，혈관증생급염증세포침윤명현증가（ P﹤0．05）；TGF-β1、CTGF표체수평명현증고（ P﹤0．05）；RGZ능개선상술병리개변，하조TGF-β1、CTGF표체（P﹤0．05）；량충제량간차이무통계학의의（P﹥0．05）。결론：재대서복막투석상관성복막섬유화모형중가용라격렬동능유효억제TGF-β1급CTGF표체。
Objective:To investigate the effect of rosiglitazone( RGZ)in prevention of peritoneal fibrosis induced by perito-neal dialysis in rats. Methods:Thirty-six SD rats were randomly divided into four groups:control group(n=9),model group(n=9),low-dose RGZ group(n=9),high-dose RGZ group(n=9). Except for control group,peritoneal catheters were implanted in another Twenty-seven rats. At the end of the study. The visceral peritoneum tissues of rats were harvested and stained with hema-toxylin-eosin( H-E)and Masson trichrome. The expression of TGF-β1 and CTGF in parietal peritoneum were detected with im-munohistochemistry assay. Results:Compared with control group,the number of peritoneal vessels,leukocytes,peritoneal thickness and the expression of TGF-β1 and CTGF were significantly higher in model group group(P﹤0. 05). Administration of RGZ resulted in preserved the number of peritoneal vessels,leukocytes and peritoneal thickness,and reduced the expression of TGF-β1 and CTGF (P﹤0. 05). But there was no significant difference between the low-dose RGZ group and the high-dose RGZ group(P﹥0. 05). Conclusion:In rat of peritoneal dialysis-related peritoneal fibrosis,administration of rosiglitazone can inhibit the expression of TGF-β1 and CTGF effective.