CAJ | 학술논문

目的：探讨三七总皂苷（panaxnonginsengsaponins，PNS）对大鼠肝缺血再灌注损伤的保护作用，并研究其对氧化应激及炎症反应相关因子的影响。方法30只健康雄性SD大鼠，体质量250~300g，随机分为假手术组（Sham组）、肝缺血再灌注组（HIR组）和三七总皂苷治疗组（PNS组），各10只。HIR组和PNS组构建肝缺血再灌注模型，PNS组术前1h予50mg/kg PNS生理盐水溶液（5mg/mL）腹腔注射，Sham组及HIR组于术前1h给予相同剂量的生理盐水腹腔注射。预处理3h后处死大鼠，检测并比较三组肝组织丙二醛（MDA）、超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）、细胞间黏附因子-1（ICAM-1）及血清肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）水平。结果 PNS组肝缺血再灌注损伤后肝组织中MDA水平、MPO活性、ICAM-1含量及血清TNF-α、IL-1β低于HIR组（P<0.05），SOD活性则高于HIR组（P<0.05）。结论三七总皂苷可通过减少氧化应激水平和炎症因子表达，减轻肝缺血再灌注损伤，达到肝脏保护作用。
목적：탐토삼칠총조감（panaxnonginsengsaponins，PNS）대대서간결혈재관주손상적보호작용，병연구기대양화응격급염증반응상관인자적영향。방법30지건강웅성SD대서，체질량250~300g，수궤분위가수술조（Sham조）、간결혈재관주조（HIR조）화삼칠총조감치료조（PNS조），각10지。HIR조화PNS조구건간결혈재관주모형，PNS조술전1h여50mg/kg PNS생리염수용액（5mg/mL）복강주사，Sham조급HIR조우술전1h급여상동제량적생리염수복강주사。예처리3h후처사대서，검측병비교삼조간조직병이철（MDA）、초양화물기화매（SOD）、수과양화물매（MPO）、세포간점부인자-1（ICAM-1）급혈청종류배사인자-α（TNF-α）、백개소-1β（IL-1β）수평。결과 PNS조간결혈재관주손상후간조직중MDA수평、MPO활성、ICAM-1함량급혈청TNF-α、IL-1β저우HIR조（P<0.05），SOD활성칙고우HIR조（P<0.05）。결론삼칠총조감가통과감소양화응격수평화염증인자표체，감경간결혈재관주손상，체도간장보호작용。
Objective To investigate the protective effect of Panaxnonginsengsaponins(PNS) on hepatic ischemia reperfusion injury(HIR) in rat and explore the mechanism. Methods Thirty SD rats were divided to three groups:sham group,HIR group,and PNS group. The model of HIR was established in HIR and PNS groups. Rats in PNS group were given 50 mg/kg PNS solution (5mg/ml) by intraperitoneal injection at 1h before surgery. Other two groups were administered with equivalent saline. After 3 h from pre-injection, all rats were sacrificed to detect liver malondialdehyde(MDA),superoxide dismutase(SOD),myeloperoxidase(MPO),intercellular adhersion molecule-1 (ICAM-1)and serum tumor necrosis factor α(TNF-α) and interleukin 1β(IL-1β). Results Compared with HIR group,the levels of liver MDA,MPO,ICAM-1 and serum TNF-α and IL-1β were higher in PNS group,while SOD was increased(P<0.05). Conclusion PNS can protect rats' liver against ischemic injury by deceasing oxidative in-jury and expression of inflammatory factors.