CAJ | 학술논문

以茶籽粕为原料，用碱法、盐法和缓冲液法提取茶籽蛋白，并测定其抗氧化活性。结果表明：盐法的茶籽蛋白得率和蛋白质含量均高于其他两种方法；盐法最佳提取条件为硫酸铵浓度0．005 mol/ L，料液比1：50，时间180 min，pH 10；在盐法最佳提取条件下，茶籽蛋白得率为5．24%，蛋白质含量为82．65%。3种提取方法所得茶籽蛋白对 DPPH 自由基、羟自由基、Fe3+都具有抗氧化活性，当盐法所得茶籽蛋白在质量浓度8．0 mg / mL 时，对 DPPH 自由基的清除率为74．78%，优于碱法和缓冲液法所得茶籽蛋白。
이다자박위원료，용감법、염법화완충액법제취다자단백，병측정기항양화활성。결과표명：염법적다자단백득솔화단백질함량균고우기타량충방법；염법최가제취조건위류산안농도0．005 mol/ L，료액비1：50，시간180 min，pH 10；재염법최가제취조건하，다자단백득솔위5．24%，단백질함량위82．65%。3충제취방법소득다자단백대 DPPH 자유기、간자유기、Fe3+도구유항양화활성，당염법소득다자단백재질량농도8．0 mg / mL 시，대 DPPH 자유기적청제솔위74．78%，우우감법화완충액법소득다자단백。
The extractions of camellia seed proteins from camellia seed meal by alkaline method, salt method and buffer method were studied, and their antioxidant activities were determined. The results showed that both the yield and protein content of camellia seed protein extracted by salt method were su-perior to other two methods. The optimal extraction conditions of salt method were obtained as follows:ammonium sulphate concentration 0. 005 mol/ L, material - liquid ratio 1: 50, time 180 min and pH 10. Under these conditions, the yield of camellia seed protein was 5. 24% and the protein content was 82. 65% . The camellia seed proteins extracted by three methods all had antioxidant abilities on DPPH radical, hydroxyl radical and Fe3 + . The scavenging rate on DPPH radical was 74. 78% when the mass concentration of camellia seed protein extracted by salt method was 8. 0 mg / mL, which was better than those extracted by alkaline method and buffer method.