CAJ | 학술논문

目的构建 pEGFP-N1-UCH-L1重组质粒，并进行鉴定。方法提取 Wistar 乳鼠大脑组织总 RNA，逆转录合成cDNA，经 PCR 扩增 UCH-L1基因，定向克隆至载体 pEGFP-N1，构建重组质粒，经测序进行鉴定。结果 UCH-L1基因重组质粒经测序鉴定证明构建正确。结论成功构建了 UCH-L1基因重组质粒，可用于神经细胞转染，进行后续研究。
목적구건 pEGFP-N1-UCH-L1중조질립，병진행감정。방법제취 Wistar 유서대뇌조직총 RNA，역전록합성cDNA，경 PCR 확증 UCH-L1기인，정향극륭지재체 pEGFP-N1，구건중조질립，경측서진행감정。결과 UCH-L1기인중조질립경측서감정증명구건정학。결론성공구건료 UCH-L1기인중조질립，가용우신경세포전염，진행후속연구。
Objective To construct and identify the eukaryotic expression vector for UCH-L1 gene. Methods Total RNA was extracted from newborn Wistar rats and reversely transcribed to cDNA,with which UCH-L1 gene was amplified by RT-PCR and cloned into the eukaryotic expression vector pEGFP-N1;the constructed recombinant plasmid was identified by restriction analysis and sequencing. Results The results of restriction analysis and sequencing proved that UCH-L1 gene is successfully inserted into plasmid pEGFP-N1. Conclusions The eukaryotic expression vector for UCH-L1 is successfully constructed,and can be used to transfect neurons in further study.