CAJ | 학술논문

目的：探讨IE mRNA和pp67 mRNA是否可以作为早期检测诊断人巨细胞病毒（HCMV）感染的标志物。方法采用荧光定量PCR的方法，在HCMV感染初诊患儿中检测IE mRNA和pp67 mRNA的表达特征。结果传统病毒DNA检测方法检测的阴性患儿中，仍然有一部分已经表达IE mRNA，而在初诊病毒DNA检测阳性的患儿中，100%表达HCMV感染后期的pp67 mRNA。结论 mRNA检测具有比DNA更高的灵敏性，应该根据不同时期病毒mRNA表达特征制定不同的治疗方案。
목적：탐토IE mRNA화pp67 mRNA시부가이작위조기검측진단인거세포병독（HCMV）감염적표지물。방법채용형광정량PCR적방법，재HCMV감염초진환인중검측IE mRNA화pp67 mRNA적표체특정。결과전통병독DNA검측방법검측적음성환인중，잉연유일부분이경표체IE mRNA，이재초진병독DNA검측양성적환인중，100%표체HCMV감염후기적pp67 mRNA。결론 mRNA검측구유비DNA경고적령민성，응해근거불동시기병독mRNA표체특정제정불동적치료방안。
Objective Human cytomegalovirus (HCMV) can induce infection in multiple organs, including genitouri-nary system, central nervous system and liver. It can even lead to serious defects and death. China, as a high-incidence area of HCMV, need employ early treatment. However, the sensitivity of present HCMV diagnosis is not satisfied. Methods In the present study, we focused on transcription level of HCMV IE and pp67 mRNA using real-time quantitative PCR. Results IE mRNA was positive even in a portion of patients who were regarded as negative by traditional DNA diagnosis method. On the other hand, pp67 mRNA that were normally expressed at the late stage of HCMV infection was 100%posi-tive in the first-visit patients who were regarded as positive using traditional DNA diagnosis method. Conclusion The pres-ent study indicated that mRNA detection based on PCR method was more sensitive compared with DNA detection for assess-ing HCMV virus infection. Finally, different treatment should be performed upon mRNA transcription profile at different in-fection stages.