CAJ | 학술논문

目的：探讨依布硒啉对大鼠急性脊髓损伤后线粒体氧化损伤、细胞色素C释放及神经细胞凋亡的影响。方法将60只健康SD大鼠按随机数字表法分为5组，每组12只。采用Allen’s法制脊髓损伤（SCI）模型，假手术组仅行椎板切除，SCI模型组行椎板切除+打击，甲基泼尼松龙组打击后给予甲基泼尼松龙腹腔注射处理（30 mg/kg），依布硒啉组打击后给予依布硒啉腹腔注射处理（10 mg/kg），生理盐水组打击后给予等体积0.1%二甲基亚砜的生理盐水溶液腹腔注射处理。术后24 h取受损脊髓，检测丙二醛（MDA）、谷胱甘肽（GSH）含量、细胞色素C表达及神经细胞凋亡情况。结果依布硒啉组较SCI模型组脊髓组织线粒体中MDA含量明显降低，GSH明显升高，Western blot检测细胞色素C的释放量减少，TUNEL法检测神经细胞凋亡数目明显减少（均P<0.01）。结论依布硒啉能够改善SCI后线粒体氧化损伤，减少线粒体细胞色素C释放，抑制神经细胞凋亡，对SCI起到保护作用。
목적：탐토의포서람대대서급성척수손상후선립체양화손상、세포색소C석방급신경세포조망적영향。방법장60지건강SD대서안수궤수자표법분위5조，매조12지。채용Allen’s법제척수손상（SCI）모형，가수술조부행추판절제，SCI모형조행추판절제+타격，갑기발니송룡조타격후급여갑기발니송룡복강주사처리（30 mg/kg），의포서람조타격후급여의포서람복강주사처리（10 mg/kg），생리염수조타격후급여등체적0.1%이갑기아풍적생리염수용액복강주사처리。술후24 h취수손척수，검측병이철（MDA）、곡광감태（GSH）함량、세포색소C표체급신경세포조망정황。결과의포서람조교SCI모형조척수조직선립체중MDA함량명현강저，GSH명현승고，Western blot검측세포색소C적석방량감소，TUNEL법검측신경세포조망수목명현감소（균P<0.01）。결론의포서람능구개선SCI후선립체양화손상，감소선립체세포색소C석방，억제신경세포조망，대SCI기도보호작용。
Objective To investigate the protective effect of Ebselen on mitochondrial damage and its influence to Cytochrome C expression and the neuronal apoptosis after spinal cord injury in rats. Methods Sixty adult SD rats were ran-domly divided into 5 groups (12 each group). Spinal cord injury model was made using Allen's method. Sham operation group received only laminectomy;SCI group received laminectomy and spinal trauma;Saline group received saline injection intraperitoneally (0.1%DMSO) after injury;methylprednisolone group received 30 mg/kg methylprednisolone injection intra-peritoneally, ebselen group received 10 mg/kg ebselen injection intraperitoneally. The malonaldehyde (MDA) and glutathi-one (GSH)level at the injured sites of the spinal cord were detected 24 hours after trauma, and the expression level of Cyto-chrome C was also observed. Finally, neuronal apoptosis was identified by TUNEL staining. Results MDA level in the Eb-selen group was significantly lower than that in the SCI group, and GSH level was significantly elevated in the Ebselen group compared with SCI group (P<0.01). Expression of Cytochrome C in Ebselen group was lower than that in SCI group shown by Western blot, and the neuronal apoptosis in Ebselen group reduced significantly too compared with SCI group (P<0.01). Conclusion Ebselen can alleviate peroxidation,prohibit expression of Cytochrome C and inhibit neuronal apoptosis,thus it shows a protective effect to experimental acute SCI.