天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2014年
8期
759-761,785
,共4页
内毒素类%脂多糖类%二异丙酚%单核细胞%肿瘤坏死因子α%髓样细胞触发受体1
內毒素類%脂多糖類%二異丙酚%單覈細胞%腫瘤壞死因子α%髓樣細胞觸髮受體1
내독소류%지다당류%이이병분%단핵세포%종류배사인자α%수양세포촉발수체1
endotoxins%lipopolysaccharides%propofol%monocytes%tumor necrosis factor-alpha%triggering receptor expressed on myeloid cells 1
目的：探讨异丙酚对内毒素诱导人单核细胞系THP-1细胞髓样细胞触发性受体-1（TREM-1）表达的影响。方法培养THP-1细胞，分成7组，分别对应：Control组，单纯无血清培养基培养；内毒素（LPS）组，单纯LPS 100μg/L刺激；Pro组，单纯加入异丙酚100μmol/L；P1组，LPS+异丙酚12.5μmol/L；P2组，LPS+异丙酚25μmol/L；P3组， LPS+异丙酚50μmol/L；P4组，LPS+异丙酚100μmol/L。观察16 h后收集细胞培养上清液采用ELISA测定肿瘤坏死因子（TNF）-α水平；收集细胞采用流式细胞术检测TREM-1蛋白表达水平；收集细胞采用实时定量PCR检测TREM-1 mRNA表达水平。结果与LPS组相比，P3组与P4组的细胞表面TREM-1蛋白表达水平明显增高（P<0.05），细胞上清液中TNF-α水平明显下降（P<0.05）；与Control组比较，LPS组TREM-1 mRNA表达明显增加（P<0.05）；与LPS组比较，P4组细胞TREM-1 mRNA表达水平降低（P<0.05）。结论异丙酚可使LPS诱导的THP-1细胞表面TREM-1蛋白表达水平增加，细胞TNF-α的释放减少，TREM-1 mRNA的表达量下降。
目的：探討異丙酚對內毒素誘導人單覈細胞繫THP-1細胞髓樣細胞觸髮性受體-1（TREM-1）錶達的影響。方法培養THP-1細胞，分成7組，分彆對應：Control組，單純無血清培養基培養；內毒素（LPS）組，單純LPS 100μg/L刺激；Pro組，單純加入異丙酚100μmol/L；P1組，LPS+異丙酚12.5μmol/L；P2組，LPS+異丙酚25μmol/L；P3組， LPS+異丙酚50μmol/L；P4組，LPS+異丙酚100μmol/L。觀察16 h後收集細胞培養上清液採用ELISA測定腫瘤壞死因子（TNF）-α水平；收集細胞採用流式細胞術檢測TREM-1蛋白錶達水平；收集細胞採用實時定量PCR檢測TREM-1 mRNA錶達水平。結果與LPS組相比，P3組與P4組的細胞錶麵TREM-1蛋白錶達水平明顯增高（P<0.05），細胞上清液中TNF-α水平明顯下降（P<0.05）；與Control組比較，LPS組TREM-1 mRNA錶達明顯增加（P<0.05）；與LPS組比較，P4組細胞TREM-1 mRNA錶達水平降低（P<0.05）。結論異丙酚可使LPS誘導的THP-1細胞錶麵TREM-1蛋白錶達水平增加，細胞TNF-α的釋放減少，TREM-1 mRNA的錶達量下降。
목적：탐토이병분대내독소유도인단핵세포계THP-1세포수양세포촉발성수체-1（TREM-1）표체적영향。방법배양THP-1세포，분성7조，분별대응：Control조，단순무혈청배양기배양；내독소（LPS）조，단순LPS 100μg/L자격；Pro조，단순가입이병분100μmol/L；P1조，LPS+이병분12.5μmol/L；P2조，LPS+이병분25μmol/L；P3조， LPS+이병분50μmol/L；P4조，LPS+이병분100μmol/L。관찰16 h후수집세포배양상청액채용ELISA측정종류배사인자（TNF）-α수평；수집세포채용류식세포술검측TREM-1단백표체수평；수집세포채용실시정량PCR검측TREM-1 mRNA표체수평。결과여LPS조상비，P3조여P4조적세포표면TREM-1단백표체수평명현증고（P<0.05），세포상청액중TNF-α수평명현하강（P<0.05）；여Control조비교，LPS조TREM-1 mRNA표체명현증가（P<0.05）；여LPS조비교，P4조세포TREM-1 mRNA표체수평강저（P<0.05）。결론이병분가사LPS유도적THP-1세포표면TREM-1단백표체수평증가，세포TNF-α적석방감소，TREM-1 mRNA적표체량하강。
Objective To evaluate the effects of propofol on TREM-1 expression in THP-1 cells stimulated by lipo-polysaccharide (LPS). Methods Cells were divided into 7 groups: (1) control group; (2) LPS group that was exposed to LPS in final concentration of 100μg/L;(3) Progroup that was incubated with 100μmol/L propofol;(4-7) groups combined 100μg/L LPS with propofol at different dose of 12.5μmol/L (P1 group), 25μmol/L (P2 group), 50μmol/L (P3 group), 100μmol/L (P4 group) respectively. After 16 hours of incubation, the TNF-α concentration in supernatants were measured by ELISA. TREM-1 expression were examined by FACS and TREM-1 mRNA were detected using qRT-PCR. Results TREM-1 on THP-1 increased significantly in group P3 and group P4 compared with LPS group (P<0.05). The concentra-tions of TNF-αin P3 and P4 (P<0.05) decreased substantially in supernatant compared with LPS group. TREM-1 mRNA transcription level in group LPS rise considerably (P < 0.05) compared to that in control group, and it dropped greatly in group P4 (P <0.05) compared with that in group LPS. Conclusion Propofol could enhance TREM-1 expression on sur-face of THP-1 stimulated by LPS. Propofol reduces TNF-αlevel in culture supernatant. And propofol may restrain TREM-1 mRNA expression.
