CAJ | 학술논문

2013年，在山东泰安采集到疑似感染黄瓜绿斑驳花叶病毒（ Cucumber green mottle mosaic virus ， CGMMV）的丝瓜样品，利用该病毒外壳蛋白（Coat protein，CP）特异引物CG-CPF/CPR对样品进行扩增并测序。所得序列（ GenBank登录号：KJ187042）经NCBI BLAST比对，与已登录的CGMMV序列的相似性均大于92．6％，确定为黄瓜绿斑驳花叶病毒。系统进化分析表明，CGMMV山东丝瓜分离物（CGMMV-SDLoofah）与辽宁分离物（ CGMMV-LN）等大多数国内CGMMV分离物同聚为一个进化组，从而进一步确定了CGMMV山东丝瓜病毒分离物为黄瓜绿斑驳花叶病毒。这是首次报道黄瓜绿斑驳花叶病毒侵染丝瓜。
2013년，재산동태안채집도의사감염황과록반박화협병독（ Cucumber green mottle mosaic virus ， CGMMV）적사과양품，이용해병독외각단백（Coat protein，CP）특이인물CG-CPF/CPR대양품진행확증병측서。소득서렬（ GenBank등록호：KJ187042）경NCBI BLAST비대，여이등록적CGMMV서렬적상사성균대우92．6％，학정위황과록반박화협병독。계통진화분석표명，CGMMV산동사과분리물（CGMMV-SDLoofah）여료녕분리물（ CGMMV-LN）등대다수국내CGMMV분리물동취위일개진화조，종이진일보학정료CGMMV산동사과병독분리물위황과록반박화협병독。저시수차보도황과록반박화협병독침염사과。
An isolate of cucumber green mottle mosaic virus ( CGMMV) was obtained from loofah show-ing typical symptoms in Taian , Shandong Province , in 2013 .The nucleotide sequence of the coat protein ( CP) of this isolate ( CGMMV-SDLoofah ) was amplified and sequenced using specific primer pair CG -CPF/CPR.The sequence analysis showed the sequence of CGMMV -SDLoofah ( GenBank accession number:KJ187042 ) had 92 .6% of identity with other isolates of CGMMV in GenBank , so it was confirmed as the CGMMV.The phylogenic analysis results indicated that CGMMV -SDLoofah could be clustered together with Liaoning isolate ( CGMMV-LN) and most other CGMMV isolates from China , which further confirmed that the CGMMV-SDLoofah was an isolate of CGMMV .This was the first report of CGMMV infecting loofah in China .