CAJ | 학술논문

目的：寻找快速、便捷有效的靶向中枢给药方法，为脑梗死治疗提供新的思路。方法线栓法制作大鼠大脑中动脉闭塞（ Middle cerebral artery occlusion，MCAO）脑缺血再灌注模型，采用ELISA法测定经鼻（ IN）和静脉（ IV）注射CGRP后30 min时，各脑区的CGRP浓度，并观察IN和IV给予CGRP对局灶性脑梗死的治疗效果。结果 IN给药组脑部各区域、颈髓和脑脊液（Cerebro-sPinal fluid，CSF）中CGRP浓度较IV组显著增高（P＜0.01），与IV组相比较，IN给药组梗死体积减小，脑血流量增加（ P＜0.01）。结论 CGRP经鼻靶向中枢给药可以避开血脑屏障阻碍，并对大鼠局灶性脑梗死有预防和保护作用。
목적：심조쾌속、편첩유효적파향중추급약방법，위뇌경사치료제공신적사로。방법선전법제작대서대뇌중동맥폐새（ Middle cerebral artery occlusion，MCAO）뇌결혈재관주모형，채용ELISA법측정경비（ IN）화정맥（ IV）주사CGRP후30 min시，각뇌구적CGRP농도，병관찰IN화IV급여CGRP대국조성뇌경사적치료효과。결과 IN급약조뇌부각구역、경수화뇌척액（Cerebro-sPinal fluid，CSF）중CGRP농도교IV조현저증고（P＜0.01），여IV조상비교，IN급약조경사체적감소，뇌혈류량증가（ P＜0.01）。결론 CGRP경비파향중추급약가이피개혈뇌병장조애，병대대서국조성뇌경사유예방화보호작용。
Objective To develoP a convenient and effective method for delivering CGRP to the central nervous system byPassing the blood-brain barrier( BBB),and to exPlore whether it had Preventive and Protective effects on cerebral in-farction in rats. Methods The MCAO model was made by nylon strand. CGRP concentration was measured IN and IV in-jection of CGRP after 30 min in different brain areas using enzyme-linked immunosorbent assay( ELISA),and exPlored the treatment IN and IV CGRP in rats with focal cerebral infarction. Results CGRP demonstrated a much higher delivery of IN than IV CGRP to the brain regions. Intranasal administration CGRP had significant Preventive and Protective effect to focal cerebral infarction which showed the brain infarction decreased and cerebral blood flow increased( P<0. 01 ). Conclusion CGRP intranasal administration targeting central administration can avoid the blood-brain barrier block,and had Preven-tive and Protective effects on the rats with focal cerebral infarction. Intranasal administration CGRP had definite Preventive and Protective effect to focal cerebral infarction in rats.