CAJ | 학술논문

以硅胶加烧石膏(占15%)作为吸附剂，正丁醇∶醋酸∶水(60∶15∶25)为展开剂，3%茚三酮乙醇溶液为显色剂，采用薄层色谱法确定合成维生素B6中酯化和酰化反应的终点。结果表明，该方法设备、操作简单，速度快，受温度影响小，灵敏度高，用于确定合成维生素B6酯化反应和酰化反应终点明显，可操作性强。
이규효가소석고(점15%)작위흡부제，정정순∶작산∶수(60∶15∶25)위전개제，3%인삼동을순용액위현색제，채용박층색보법학정합성유생소B6중지화화선화반응적종점。결과표명，해방법설비、조작간단，속도쾌，수온도영향소，령민도고，용우학정합성유생소B6지화반응화선화반응종점명현，가조작성강。
Used silica gel and bassanite (15%) as absorbent, n-butyl alcohol:acetic acid:water(60:15:25) as developing solvent, 3%ninhydrin ethanol solution as color-developing agent,the end point of esterification and acylation reaction in synthesis of vitamine B6 was determined by thin-layer chromatography. The test result showed that this method was easy, rapid, simple equipment, little tem-perature influence, high sensitive and operability. The esterification and acylation reaction endpoint was clear.