中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
13期
36-37,38
,共3页
银杏内酯%超高效液相色谱%树脂分离%参银复智
銀杏內酯%超高效液相色譜%樹脂分離%參銀複智
은행내지%초고효액상색보%수지분리%삼은복지
ginkgolide%UPLC%resin separating%Shenyinfuzhi Preparation
目的：寻找从参银复智制剂组方君药银杏叶中提取、分离、检测有效成分白果内酯和银杏内酯 A，B，C的快速简便的方法。方法采用超高效液相色谱法检测含量，色谱柱为Waters BEH C18柱（50 mm ×2.1 mm，1.7μm），流动相为甲醇-四氢呋喃-水（25：5：70），流速为0.3 mL/min，柱温为30℃，蒸发光散射检测器( ELSD )。银杏叶采用7倍70%乙醇回流提取2次，每次3 h，合并提取液，减压回收乙醇，加3倍量水使沉淀，过滤，滤液分别上大孔吸附树酯D101，AB-8，D201及聚酰胺树脂，考察洗脱剂的浓度和用量。结果选择聚酰胺树脂，2倍水洗后，用2～3倍树脂床体积的10%乙醇洗脱，收集洗脱液，减压浓缩干燥，检测含量，银杏萜类内酯的含量都在35%以上，有效部位得率在90%以上。结论该方法简便、快速、回收率高，为银杏叶中内酯类物质的提取、分离、检测提供了一种新方法。
目的：尋找從參銀複智製劑組方君藥銀杏葉中提取、分離、檢測有效成分白果內酯和銀杏內酯 A，B，C的快速簡便的方法。方法採用超高效液相色譜法檢測含量，色譜柱為Waters BEH C18柱（50 mm ×2.1 mm，1.7μm），流動相為甲醇-四氫呋喃-水（25：5：70），流速為0.3 mL/min，柱溫為30℃，蒸髮光散射檢測器( ELSD )。銀杏葉採用7倍70%乙醇迴流提取2次，每次3 h，閤併提取液，減壓迴收乙醇，加3倍量水使沉澱，過濾，濾液分彆上大孔吸附樹酯D101，AB-8，D201及聚酰胺樹脂，攷察洗脫劑的濃度和用量。結果選擇聚酰胺樹脂，2倍水洗後，用2～3倍樹脂床體積的10%乙醇洗脫，收集洗脫液，減壓濃縮榦燥，檢測含量，銀杏萜類內酯的含量都在35%以上，有效部位得率在90%以上。結論該方法簡便、快速、迴收率高，為銀杏葉中內酯類物質的提取、分離、檢測提供瞭一種新方法。
목적：심조종삼은복지제제조방군약은행협중제취、분리、검측유효성분백과내지화은행내지 A，B，C적쾌속간편적방법。방법채용초고효액상색보법검측함량，색보주위Waters BEH C18주（50 mm ×2.1 mm，1.7μm），류동상위갑순-사경부남-수（25：5：70），류속위0.3 mL/min，주온위30℃，증발광산사검측기( ELSD )。은행협채용7배70%을순회류제취2차，매차3 h，합병제취액，감압회수을순，가3배량수사침정，과려，려액분별상대공흡부수지D101，AB-8，D201급취선알수지，고찰세탈제적농도화용량。결과선택취선알수지，2배수세후，용2～3배수지상체적적10%을순세탈，수집세탈액，감압농축간조，검측함량，은행첩류내지적함량도재35%이상，유효부위득솔재90%이상。결론해방법간편、쾌속、회수솔고，위은행협중내지류물질적제취、분리、검측제공료일충신방법。
Objective To seek the rapid and simple method for extracting, separating and detecting the effective component bilobalide and ginkgolide A, B and C from the monarch drug Ginkgo biloba leaves in Shenyinfuzhi Preparation. Methods The UPLC method was adopted for detecting the content. The chromatographic column was the Waters BEH C18 column ( 50 mm × 2. 1 mm, 1. 7 μm ) with the mobile phase of methanol-tetrahydrofuran-water ( 25:5:70 ) at a flow rate of 0. 3 mL/min. The column temperature was set at 30℃. The detection was carried out with the ELSD detector. The Ginkgo biloba leaves were extracted with 7 times 70%ethanol, refluxed twice, 3 h each time, the extract solution was combined, decompressed for recycling ethanol, adding 3 times the amount of water to pre-cipitate the impurity, and then filtrated by the macroporous adsorption resin D101, AB-8, D201 and polyamide resin respectively. The concentration and the use amount of the eluent were investigated. Results Polyamide resin was selected, after washing by 2 times of water, 10% ethanol elution with the 2-3 times of resin bed volume was performed. Then the eluent was collected, depressed, concentrat-ed and dried. The content was detected. The content of ginkgo terpene lactones was more than 35% and the yield rate of the effective parts was more than 90%. Conclusion This method is simple and rapid with high recovery rate, which provides a new method for ex-tracting, separating and detecting ginkgo terpene lactones from Ginkgo biloba leaves.
