CAJ | 학술논문

目的：研究 EphA2基因在大鼠白内障晶状体中的表达水平变化的情况。方法选用18只SD大鼠的36个晶状体，分为3组，每组6只，分别为2月龄透明晶状体组（青年组）、12月龄透明晶状体组（老年组）和12月龄白内障晶状体组（白内障组）。采用实时荧光定量 PCR 和Western blot检测青年组、老年组和白内障组晶状体中EphA2基因和蛋白的表达水平。结果 Western blot检测结果显示EphA2蛋白在白内障组中表达水平明显低于老年组（t＝10.952，P＝0.000）；而老年组比青年组EphA2蛋白表达水平呈轻度的升高（t＝-4.456，P＝0.001）。Q-PCR结果显示，白内障组中的EphA2 mRNA表达水平较老年组呈明显的升高（t＝-4.518，P＝0.001）；老年组中的EphA2 mRNA表达水平较青年组呈轻度的降低（t＝8.237，P＝0.000）。结论 EphA2蛋白表达水平下降与大鼠白内障发生密切相关。随着大鼠年龄增加，EphA2蛋白表达水平呈轻度的升高。
목적：연구 EphA2기인재대서백내장정상체중적표체수평변화적정황。방법선용18지SD대서적36개정상체，분위3조，매조6지，분별위2월령투명정상체조（청년조）、12월령투명정상체조（노년조）화12월령백내장정상체조（백내장조）。채용실시형광정량 PCR 화Western blot검측청년조、노년조화백내장조정상체중EphA2기인화단백적표체수평。결과 Western blot검측결과현시EphA2단백재백내장조중표체수평명현저우노년조（t＝10.952，P＝0.000）；이노년조비청년조EphA2단백표체수평정경도적승고（t＝-4.456，P＝0.001）。Q-PCR결과현시，백내장조중적EphA2 mRNA표체수평교노년조정명현적승고（t＝-4.518，P＝0.001）；노년조중적EphA2 mRNA표체수평교청년조정경도적강저（t＝8.237，P＝0.000）。결론 EphA2단백표체수평하강여대서백내장발생밀절상관。수착대서년령증가，EphA2단백표체수평정경도적승고。
Objective To investigate the relationship between expression of EphA2 and cataract in rat. Methods Thirty-six lenses from SD rats were allocated into three groups:clear younger, older and cataractous lenses. Expression of EphA2 in the lenses of younger, older, and cataractous was detected by quantitative real time RT-PCR and western blot. Results Western blot analysis showed that EphA2 protein in rat cataractous lenses was lower compared to clear older lenses (t=10.952, P=0.000). The older lenses slightly higher in the level expression of EphA2 protein compared to younger lenses (t=-4.456, P=0.001). Real time RT-PCR analysis showed that EphA2 mRNA expression in rat cataractous lenses was higher than clear older lenses (t=-4.518, P=0.001). However, EphA2 mRNA expression in older lenses was lower than younger lenses (t=8.237, P=0.000). Conclusions Lower level expression of EphA2 was related to cataract in rats. The expression level of EphA2 protein in rats’ lenses was affected by age.