CAJ | 학술논문

目的：建立稳定高表达microRNA-218膀胱癌T24细胞系。方法构建pHBLV-U6-ZsGreen-Puro质粒，制备慢转录病毒载体pHBLV-U6-ZsGreen-Puro-miR-218和pHBLV-U6-ZsGreen-Puro-vector转染膀胱癌T24细胞系，用嘌呤霉素筛选并荧光显微镜和qRT-PCR检测。结果转染的细胞在荧光显微镜下呈现出绿色荧光，实时荧光定量PCR（qRT-PCR）显示实验组细胞microRNA-218表达水平比对照组升高250倍。结论该实验成功建立了稳定高表达MicroRNA-218膀胱癌T24细胞系，为进一步研究microRNA-218在膀胱癌中的功能奠定了基础。
목적：건립은정고표체microRNA-218방광암T24세포계。방법구건pHBLV-U6-ZsGreen-Puro질립，제비만전록병독재체pHBLV-U6-ZsGreen-Puro-miR-218화pHBLV-U6-ZsGreen-Puro-vector전염방광암T24세포계，용표령매소사선병형광현미경화qRT-PCR검측。결과전염적세포재형광현미경하정현출록색형광，실시형광정량PCR（qRT-PCR）현시실험조세포microRNA-218표체수평비대조조승고250배。결론해실험성공건립료은정고표체MicroRNA-218방광암T24세포계，위진일보연구microRNA-218재방광암중적공능전정료기출。
Objective To establish stable and high bladder cancer T24 cell line overexpressing microRNA-218 for the further studies on the function of microRNA-218 in bladder cancer. Methods pHBLV-U6-ZsGreen-Puro plasmids and lentivirus of pHBLV-U6-ZsGreen-Puro-vector or pHBLV-U6-ZsGreen-Puro-miR-218 was constructed. Both lentivirus were transfected into bladder cancer T24 cell line respectively. After drug screening with puromycin,the stable cell lines were detected by fluorescence microscope and qRT-PCR. Results Green fluorescence in transfected cell lines was observed by fluorescence microscopy. qRT-PCR showed microRNA-218 expression level of experimental group was 250 folds higher than that of control group. Conclusion The stable and high bladder cancer T24 cell line overexpressing microRNA-218 was established, which provides foundations to further study on the functions of microRNA-218 in bladder cancer.