CAJ | 학술논문

目的：建立荔之咽滴丸中高车前苷含量的高效液相色谱（HPLC）测定方法，为荔之咽滴丸的质量控制提供依据。方法色谱柱：Hedera ODS-2（250.0 mm×4.6 mm，5μm）；流动相：甲醇∶0.5%冰醋酸水溶液（50∶50）；柱温：30℃；流速：1.0 mL/min；检测波长：335 nm；进样量：10μL。结果高车前苷在0.20~2.52μg时呈良好的线性关系，相关系数（r）＝0.9998；平均回收率为99.80%，其相对标准偏差（RSD）为2.35%。结论 HPLC用于测定荔之咽滴丸中高车前苷含量，方法简便、准确、专属性强、重复性好，能起到控制荔之咽滴丸中高车前苷含量的作用。
목적：건립려지인적환중고차전감함량적고효액상색보（HPLC）측정방법，위려지인적환적질량공제제공의거。방법색보주：Hedera ODS-2（250.0 mm×4.6 mm，5μm）；류동상：갑순∶0.5%빙작산수용액（50∶50）；주온：30℃；류속：1.0 mL/min；검측파장：335 nm；진양량：10μL。결과고차전감재0.20~2.52μg시정량호적선성관계，상관계수（r）＝0.9998；평균회수솔위99.80%，기상대표준편차（RSD）위2.35%。결론 HPLC용우측정려지인적환중고차전감함량，방법간편、준학、전속성강、중복성호，능기도공제려지인적환중고차전감함량적작용。
Objective To establish determination method of homoplantaginin content in the Lizhiyan dropping pills by high performance liquid chromatography(HPLC),in ordetr to provide a basis for the quality control of Lizhiyan dropping pills. Methods The chromatographic column:Hedera ODS-2(250.0 mm × 4.6 mm,5μm);mobile phase:methanol∶0.5%glacial acetic acid(50∶50);column temperature:30℃;flow rate:1.0 mL/min;detection wavelength:335 nm;injection volume:10μL. Results Homoplantaginin showed a good linear relationship when the injection volume was within 0.20-2.52 μg with the correlation coeffi-cient of 0.999 8;the average recovery rate was 99.8%,and the relative standard deviation (RSD) was 2.35%. Conclusion This method is simple,accurate,specific and repeatable,and it has contribution to control the content of homoplantaginin in the Lizhiyan dropping pills.