中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2014年
22期
26-28
,共3页
曹聪明%丁红涛%李妙荣%孙晓海
曹聰明%丁紅濤%李妙榮%孫曉海
조총명%정홍도%리묘영%손효해
妊娠期糖尿病%胰岛素受体底物-1%葡萄糖转运蛋白4%胰岛素
妊娠期糖尿病%胰島素受體底物-1%葡萄糖轉運蛋白4%胰島素
임신기당뇨병%이도소수체저물-1%포도당전운단백4%이도소
Gestational diabetes mellitus%Insulin receptor substrate-1%Glucose transporter 4%Insulin
目的：探讨妊娠期糖尿病患者胎盘组织中IRS-1和GLUT4的表达。方法选取2012年3月~2014年3月本院产科门诊收治的住院分娩孕妇共83例,按照检查结果将其分为妊娠期糖尿病组(GDM组)(n=43)和正常足月妊娠组(对照组)(n=40)。检测两组孕妇的各临床指标(身高、血压、体重以及空腹血糖、空腹胰岛素、总胆固醇、三酰甘油等),同时采用逆转录-聚合酶链式反应(RT-PCR)方法测定患者胎盘组织中的IRS-1和GLUT4的表达水平。结果 GDM组的空腹血糖、空腹胰岛素、总胆固醇、三酰甘油等指标水平均高于对照组(P<0.05)。 GDM组的HOMA-IR高于对照组,GDM组的HOMA-ISI、HOMA-β%低于对照组(P<0.05)。IRS-1在两组胎盘组织中的表达水平比较,差异有统计学意义(P<0.05)；GLUT4在两组胎盘组织胞质中的表达水平的比较,差异无统计学意义(P>0.05),在胎盘组织细胞膜中的表达水平比较,差异有统计学意义(P<0.05)。结论 IRS-1、GLUT4作为胰岛素信号通路中的信号转导分子,其在胰岛素抵抗环节中发挥重要作用。
目的：探討妊娠期糖尿病患者胎盤組織中IRS-1和GLUT4的錶達。方法選取2012年3月~2014年3月本院產科門診收治的住院分娩孕婦共83例,按照檢查結果將其分為妊娠期糖尿病組(GDM組)(n=43)和正常足月妊娠組(對照組)(n=40)。檢測兩組孕婦的各臨床指標(身高、血壓、體重以及空腹血糖、空腹胰島素、總膽固醇、三酰甘油等),同時採用逆轉錄-聚閤酶鏈式反應(RT-PCR)方法測定患者胎盤組織中的IRS-1和GLUT4的錶達水平。結果 GDM組的空腹血糖、空腹胰島素、總膽固醇、三酰甘油等指標水平均高于對照組(P<0.05)。 GDM組的HOMA-IR高于對照組,GDM組的HOMA-ISI、HOMA-β%低于對照組(P<0.05)。IRS-1在兩組胎盤組織中的錶達水平比較,差異有統計學意義(P<0.05)；GLUT4在兩組胎盤組織胞質中的錶達水平的比較,差異無統計學意義(P>0.05),在胎盤組織細胞膜中的錶達水平比較,差異有統計學意義(P<0.05)。結論 IRS-1、GLUT4作為胰島素信號通路中的信號轉導分子,其在胰島素牴抗環節中髮揮重要作用。
목적：탐토임신기당뇨병환자태반조직중IRS-1화GLUT4적표체。방법선취2012년3월~2014년3월본원산과문진수치적주원분면잉부공83례,안조검사결과장기분위임신기당뇨병조(GDM조)(n=43)화정상족월임신조(대조조)(n=40)。검측량조잉부적각림상지표(신고、혈압、체중이급공복혈당、공복이도소、총담고순、삼선감유등),동시채용역전록-취합매련식반응(RT-PCR)방법측정환자태반조직중적IRS-1화GLUT4적표체수평。결과 GDM조적공복혈당、공복이도소、총담고순、삼선감유등지표수평균고우대조조(P<0.05)。 GDM조적HOMA-IR고우대조조,GDM조적HOMA-ISI、HOMA-β%저우대조조(P<0.05)。IRS-1재량조태반조직중적표체수평비교,차이유통계학의의(P<0.05)；GLUT4재량조태반조직포질중적표체수평적비교,차이무통계학의의(P>0.05),재태반조직세포막중적표체수평비교,차이유통계학의의(P<0.05)。결론 IRS-1、GLUT4작위이도소신호통로중적신호전도분자,기재이도소저항배절중발휘중요작용。
Objective To explore the Expression of IRS-1,GLUT4 in placenta of patients with gestational diabetes mellitus. Methods 83 pregnant women treated in outpatient department in ourhosptal from March 2012 to March 2014 obstetric outpatient were selected and the gestational diabetes mellitus (GDM) group (n=43)and the The normal full-term pregnancy group (the control group) (n=40) according to examination results.Different clinical indicators (height, blood pressure,weight,and fasting blood glucose, fasting insulin,total cholesterol,triglyceride,etc.), in the two groups were detected,expression level of IRS-1 and GLUT4 of placenta in patients were determined by reverse transcription poly-merase chain reaction (RT-PCR) methods in the two groups. Results The level of fasting glucose,fasting insulin,total cholesterol,and triglycerides and so on in the GDM group were higher than those in the control group (P<0.05).HOMA-IR of the GDM group was higher than that in the control group,HOMA-ISI,HOMA-β% the GDM group was lower than that in the control group (P<0.05).There was a statistical difference of IRS-1 expression level of placenta in the two groups (P<0.05);there was no statistical difference of GLUT4 expression level of cytoplasm in placenta of the two groups (P>0.05),there was a statistical difference of GLUT4 expression level of Cell membrane in placenta of the two groups (P<0.05). Conclusion As a signal transduction molecule in the insulin signaling pathway,IRS-1,GLUT4 can play an im-portant role in insulin resistance.
