广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2014年
15期
158-161
,共4页
高效液相色谱法%天麻素注射液%含量测定%有关物质
高效液相色譜法%天痳素註射液%含量測定%有關物質
고효액상색보법%천마소주사액%함량측정%유관물질
HPLC%gastrodin injection%content determination%related substances
建立了高效液相色谱法测定天麻素注射液的含量及有关物质。色谱柱为依利特ODS C18柱（250 mm ×4.6 mm，5μm）；以水-乙腈-磷酸（97.4：2.5：0.1）为流动相；检测波长为220 nm，流速为1.0 mL/min。结果表明：天麻素含量浓度在0.004～0.40 mg/min范围内与峰面积具有良好的线性关系（ r2＝0.9999），平均回收率为100.24％， RSD为0.13％。该方法灵敏，精密度高，结果准确可靠，可用于天麻素注射液的质量控制。
建立瞭高效液相色譜法測定天痳素註射液的含量及有關物質。色譜柱為依利特ODS C18柱（250 mm ×4.6 mm，5μm）；以水-乙腈-燐痠（97.4：2.5：0.1）為流動相；檢測波長為220 nm，流速為1.0 mL/min。結果錶明：天痳素含量濃度在0.004～0.40 mg/min範圍內與峰麵積具有良好的線性關繫（ r2＝0.9999），平均迴收率為100.24％， RSD為0.13％。該方法靈敏，精密度高，結果準確可靠，可用于天痳素註射液的質量控製。
건립료고효액상색보법측정천마소주사액적함량급유관물질。색보주위의리특ODS C18주（250 mm ×4.6 mm，5μm）；이수-을정-린산（97.4：2.5：0.1）위류동상；검측파장위220 nm，류속위1.0 mL/min。결과표명：천마소함량농도재0.004～0.40 mg/min범위내여봉면적구유량호적선성관계（ r2＝0.9999），평균회수솔위100.24％， RSD위0.13％。해방법령민，정밀도고，결과준학가고，가용우천마소주사액적질량공제。
The HPLC method for determination of gastrodin injection and its related substances was established.The method was performed with C18 (250 ×4.6 mm, 5 μm) as the column.The mobile phase was consisted of water -acetonitrile-phosphoric acid (97.4:2.5:0.1) with a flow rate at 0.1 ml/min.The detection wavelength was 220 nm.The calibration cure of grastrodin was linear in the concentration range of 0.004 ~0.40 mg/mL ( r2 =0.9999 ).The average recovery rate was 100.24%and RDS was 0.13%.This method was sensitive , accurate and specific , which can be used for the quality control of the products.
